水稻种子耐厌氧萌发全基因组关联分析  

Genome Wide Association Study of Anaerobic Germination Tolerance in Seeds of Rice Accessions

作　　者：刘利成[1,2] 李小湘[2] 黎用朝[2] 潘孝武[2] 闵军[2] 刘三雄[2] 刘文强[2] 胡敏 段永红[2] 余亚莹 张海清[1] LIU Li-cheng;LI Xiao-xiang;LI Yong-chao;PAN Xiao-wu;MIN Jun;LIU San-xiong;LIU Wen-qiang;HU Min;DUAN Yong-hong;YU Ya-ying;ZHANG Hai-qing(College of Agriculture,Hunan Agricultural University,Changsha 410128;Hunan Rice Research Institute,Hunan Academy of Agricultural Science/Key Laboratory of Indica Rice Genetics and Breeding in the Middle and Lower Reaches of Yangtze River Valley,Ministry of Agriculture,Changsha 410125)

机构地区：[1]湖南农业大学农学院,长沙410128 [2]湖南省农业科学院水稻研究所/农业部长江中下游籼稻遗传育种重点实验室,长沙410125

出　　处：《植物遗传资源学报》2021年第6期1644-1650,共7页Journal of Plant Genetic Resources

基　　金：国家现代农业产业技术体系建设专项(CARS-01-14)。

摘　　要：本研究通过解析水稻芽期耐淹性的遗传基础,挖掘相关QTL,筛选优良单株,为分子标记辅助育种提供优异种质和理论支撑。选取318份国内外水稻种质资源,在正常有氧和厌氧条件下测定种子萌发期的胚芽鞘长度,并计算相对胚芽鞘长度,通过整合基因型和表型数据进行全基因组关联分析。318份水稻种质间相对胚芽鞘长度差异明显,相对胚芽鞘长度的平均值为-0.238,中位值为-0.342,最低值为-0.923,最高值为3.069。全基因组关联分析共鉴定到83个SNP与种子耐厌氧萌发性状显著相关。通过合并距离较近的SNP,共鉴定到27个耐厌氧萌发QTL,3个QTL与已报道的耐厌氧萌发QTL位置相近。其中qAG4-2与耐厌氧萌发性状的关联度较高,共有20个候选基因位于qAG4-2内,其中1个基因在厌氧条件下相对于正常有氧条件上调表达,2个基因下调表达。本研究挖掘的控制水稻耐厌氧萌发的QTL及优异种质资源将为水稻耐厌氧育种提供重要支撑。By dissecting genetic basis and identifying QTL related to anaerobic germination related traits in rice,and detecting the elite rice lines carrying favorable alleles,this study provided excellent germplasm for molecular marker-assisted(MAS)breeding.The seeds of 318 rice lines were germinated for measuring coleoptile length under normal and anaerobic conditions.QTL associated with anaerobic germination(AG)tolerance was determined by GWAS and candidate genes were analyzed.The broad variation of AG tolerance was observed among rice accessions ranged from-0.923 to 3.069,with an average of-0.238,a median of-0.342.A total of 27 QTL revealed by 83 SNPs were detected associating with AG tolerance.The QTL locus qAG4-2 was selected for analysis and 20 candidate genes in the target interval were annotated.Among them,one gene was up-regulated and two genes were down-regulated under normal condition compared to anaerobic condition.The excellent germplasm resources and the QTL loci associating with anaerobic germination were valuable in rice breeding.

关 键 词：水稻 厌氧萌发 全基因组关联分析 

分 类 号：S511[农业科学—作物学]