S1P/S1PR1通路调节补体B因子的表达对食管鳞状细胞癌细胞增殖和迁移的影响  

Effects of S1P/S1PR1 pathway regulating expression of complement factor B on proliferation and migration of esophageal squamous cell carcinoma cells

作　　者：徐凤敏 陈昱伶 任玲 蒙春梅 李倩倩[1] 胡为民[1] XU Feng-Min;CHEN Yu-Ling;REN Ling;MENG Chun-Mei;LI Qian-Qian;HU Wei-Min(Department of Microbiology&Immunology,North Sichuan Medical College,Nanchong 637100,China)

机构地区：[1]川北医学院微生物学与免疫学教研室,南充637100

出　　处：《中国免疫学杂志》2021年第19期2364-2369,共6页Chinese Journal of Immunology

基　　金：四川省科技厅重点科技自筹项目(2011JYZ023);南充市市校合作科研专项(NSMC20170404,20SXQT0332)资助。

摘　　要：目的:探讨1-磷酸鞘氨醇(S1P)/1型1-磷酸鞘氨醇受体(S1PR1)通路调节补体B因子(CFB)的表达对食管鳞状细胞癌(ESCC)细胞增殖和迁移的影响。方法:采用RT-qPCR检测食管鳞癌Eca109、TE-1、KYSE150细胞中S1PR1、CFB的表达量,以及在Eca109细胞中过表达S1PR1对CFB表达的影响;采用过表达和小干扰RNA(siRNA),用Lipofectamine^(TM)2000将S1PR1-EGFP过表达载体和CFB siRNA共转染Eca109细胞,CCK8法和划痕实验检测细胞的增殖和迁移;CFB siRNA转染TE-1细胞,检测细胞的增殖和迁移。结果:在低表达S1PR1和CFB的Eca109细胞中过表达S1PR1,结果显示诱导CFB表达上调,促进细胞的增殖和迁移,并且干扰CFB表达可抑制上述作用;在高表达S1PR1和CFB的TE-1细胞中敲低CFB的表达,TE-1细胞增殖和迁移受到明显抑制。结论:S1P/S1PR1通路能诱导CFB表达,促进食管鳞癌细胞的增殖和迁移,CFB可能在ESCC的发生发展中发挥重要作用。Objective:To investigate the effects of sphingosine 1-phosphate(S1P)/sphingosine 1-phosphate receptor 1(S1PR1)pathway regulating the expression of complement factor B(CFB)on proliferation and migration of esophageal squamous cell carcinoma cells.Methods:S1PR1 and CFB mRNA expression in esophageal squamous cell carcinoma(ESCC)cell lines Eca109,TE-1 and KYSE150,and CFB mRNA expression in S1PR1-overexpressing Eca109 cells was detected by RT-qPCR.Using overexpression and small interference RNA(siRNA)methods,S1PR1-EGFP overexpression vector and CFB siRNA were cotransfected into Eca109 cells by Lipofectamine^(TM)2000.The proliferation and migration of Eca109 cells were detected by CCK8 and wound healing assay,respectively.CFB siRNA was transfected into TE-1 cells,and the proliferation and migration were detected.Results:Overexpression of S1PR1 in Eca109 cells which were low expression of S1PR1 and CFB up-regulated CFB expression,and promoted the proliferation and migration of Eca109 cells.Knockdown of CFB in S1PR1-overexpressing Eca109 cells inhibited these effects.Knockdown of CFB in TE-1 cells which were high expression of S1PR1 and CFB inhibited the proliferation and migration of TE-1 cells.Conclusion:S1P/S1PR1 pathway inducing the expression of CFB promotes the proliferation and migration of ESCC cells.CFB might play an important role in ESCC progression.

关 键 词：1型1-磷酸鞘氨醇受体 补体B因子 食管鳞状细胞癌 增殖 迁移 

分 类 号：R735.[医药卫生—肿瘤]