过表达NAMPT脐带间充质干细胞外泌体的抗衰老作用  

The effect of overexpression of NAMPT of human umbilical cord mesenchymal stem cell exosomes on aging

作　　者：周琦琦 孙振[1,2] 刘洋洋 李华玲[3] 崔恒宓[1,2] ZHOU Qiqi;SUN Zhen;LIU Yangyang;LI Hualing;CUI Hengmi(Institute of Epigenetics and Epigenomics/College of Animal and Science,Yangzhou University,Yangzhou 225009,China;Jiangsu Innovation Institute for Biomedicine,Nanjing 211102,China;College of Medicine,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学动物科学与技术学院/表观遗传学及表观基因组学研究所,江苏扬州225009 [2]江苏易诺维生物医学研究院,南京211102 [3]扬州大学医学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2021年第4期1-6,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金面上项目(81773013);国家重点研发计划项目(2016YFC1303604)。

摘　　要：NAMPT是NAD+补救合成途径的限速酶,具有抗衰老作用。外泌体是一种包含蛋白质、miRNA、mRNA、lncRNA等内容物的天然内源性载体。利用慢病毒方法构建NAMPT过表达的人脐带间充质干细胞系,获得过表达NAMPT脐带间充质干细胞外泌体,随后通过与人皮肤成纤维细胞共培养和饲喂秀丽隐杆线虫,研究过表达NAMPT脐带间充质干细胞外泌体对其抗衰老的影响。结果表明:基因修饰后脐带间充质干细胞及其外泌体内NAMPT蛋白含量显著升高,并能促进人皮肤成纤维细胞增殖和延缓人皮肤成纤维细胞衰老;同时能延长秀丽隐杆线虫寿命和增加秀丽隐杆线虫抗氧化能力。这一研究提示过表达NAMPT基因的间充质干细胞外泌体具有一定的抗衰老作用。NAMPT is the rate limiting enzyme of NAD+salvage pathway and has anti-aging effect. Exosomes are natural endogenous vectors containing protein, miRNA, mRNA and lncRNA. A human umbilical cord mesenchymal stem cell(hUCMSC) line overexpressing NAMPT was constructed by lentiviral transfection, and the exosomes was isolated from hUCMSCs overexpressing NAMPT. Then, the anti-aging effects of the exosomes derived from hUCMSCs overexpressing NAMPT were studied by co-culturing with human skin fibroblasts(HSF) and feeding Caenorhabditis elegans. The results showed that NAMPT transgene significantly increase NAMPT protein levels in hUCMSCs and their exosomes. And these exosomes can promote the proliferation and delay the aging of human skin fibroblasts. It also can prolong the life span of C.elegans and increase the antioxidant capacity of C.elegans. These preliminary results indicate that the exosomes of mesenchymal stem cells overexpressing the NAMPT gene have an anti-aging effect.

关 键 词：人脐带间充质干细胞 外泌体 NAMPT 人皮肤成纤维细胞 秀丽隐杆线虫 

分 类 号：R457.7[医药卫生—治疗学]