甲状腺乳头状癌病人血清微小RNA-451的检测及其诊断价值的研究  

The expression of microRNA-451 in serum of papillary thyroid carcinoma and its diagnostic value

作　　者：马静[1] 张楠[1] 马斌林[1] MA Jing;ZHANG Nan;MA Binlin(Department of Breast and Head-Neck Surgery,the Affiliated Tumor Hospital,Xin Jiang Medical University,Urumqi 830000,China)

机构地区：[1]新疆医科大学附属肿瘤医院乳腺头颈外科,乌鲁木齐830000

出　　处：《临床外科杂志》2021年第10期953-956,共4页Journal of Clinical Surgery

基　　金：新疆医科大学科研创新基金项目(XYDCX201682)。

摘　　要：目的探讨甲状腺乳头状癌病人血清中微小RNA-451的表达及其对甲状腺乳头状癌的诊断意义。方法36例甲状腺乳头状癌病人为研究组,37例甲状腺良性肿瘤病人为对照组,实时荧光定量PCR检测两组病人血清中微小RNA-451的相对表达量,比较两组之间血清微小RNA-451的表达差异,分析甲状腺乳头状癌病人血清微小RNA-451的表达与不同临床病理特征之间的关系,通过ROC工作曲线分析血清微小RNA-451对甲状腺乳头状癌的诊断意义。结果甲状腺乳头状癌病人血清微小RNA-451的表达为(1.09±0.27),明显低于甲状腺良性肿瘤病人的(1.31±0.23);甲状腺乳头状癌淋巴结有转移病人的血清微小RNA-451表达为(0.92±0.17),明显低于淋巴结无转移病人的(1.28±0.22),差异均有统计学意义(P<0.05)。通过ROC曲线分析,血清微小RNA-451诊断甲状腺乳头状癌的曲线下面积(AUC)为0.720;在判断其对淋巴结转移诊断作用时的AUC为0.873。结论甲状腺乳头状癌病人血清中的微小RNA-451呈低表达,尤其是在淋巴结有转移病人中的表达更低,提示其对甲状腺乳头状癌的术前诊断及淋巴结转移状态有一定的意义。Objective To investigate the expression of microrna-451 in serum of patients with papillary thyroid carcinoma and its diagnostic significance.Methods Thirty-six patients with papillary thyroid carcinoma were selected as the study group,and thirty-seven patients with benign thyroid tumor were selected as the control group.The relative expression of serum microrna-451 in the two groups was detected by real-time fluorescent quantitative PCR.The expression difference of serum microrna-451 between the two groups was compared,and the relationship between the expression of serum microrna-451 and different clinicopathological features in patients with papillary thyroid carcinoma was analyzed.The diagnostic value of serum microrna-451 in papillary thyroid carcinoma was analyzed by curve analysis.Results The expression of serum microrna-451 in patients with papillary thyroid carcinoma was significantly lower than that in patients with benign thyroid tumor(1.09±0.27 vs 1.31±0.23);The expression of serum microrna-451 in patients with lymph node metastasis of papillary thyroid carcinoma was significantly lower than that in patients without lymph node metastasis(0.92±0.17 vs 1.28±0.22,P<0.001),the differences were statistically significant;By ROC curve analysis,the AUC of serum microrna-451 in the diagnosis of papillary thyroid carcinoma was 0.720,and 0.873 in the diagnosis of lymph node metastasis.Conclusion The expression of microrna-451 in serum of patients with papillary thyroid carcinoma is low,especially in patients with lymph node metastasis,which indicates that it has certain diagnostic significance for preoperative diagnosis and lymph node metastasis of papillary thyroid carcinoma.

关 键 词：微小RNA-451 甲状腺乳头状癌 淋巴结转移 

分 类 号：R736.1[医药卫生—肿瘤]