化学发光免疫分析法检测尿液中N^(1),N^(12)-二乙酰精胺含量的研究  

Study on Determination of N^(1),N^(12)-Diacetylspermine in Urine by Chemiluminescence Immunoassay

作　　者：杨梦瑶 吴雨晴 刘辰辰 郭宇[2] 刘俊伟[1] YANG Mengyao;WU Yuqing;LIU Chenchen;GUO Yu;LIU Junwei(Tianjin International Joint Academy of Biomedicine,Tianjin 300457,China;College of Pharmacy,Nankai University,Tianjin 300350,China)

机构地区：[1]天津国际生物医药联合研究院,天津300457 [2]南开大学药学院,天津300350

出　　处：《天津科技》2021年第11期25-28,共4页Tianjin Science & Technology

基　　金：国家重点研发计划项目“中和性抗体干预病毒入侵过程的结构生物学基础”(2018YFE0200402)。

摘　　要：N^(1),N^(12)-二乙酰精胺是精胺的二乙酰化衍生物。有研究表明,包括结直肠癌、乳腺癌、卵巢癌、肝癌、泌尿生殖系统癌和非小细胞肺癌在内的多种癌症患者尿液中的N^(1),N^(12)-二乙酰精胺水平显著高于正常人。在制备出抗N^(1),N^(12)-二乙酰精胺多克隆抗体的基础上,通过优化包被原、封闭液、抗体稀释倍数等参数,建立了可用于检测人体尿液样本中N^(1),N^(12)-二乙酰精胺的化学发光免疫分析方法。该方法的最低检出限为(0.19±0.04)ng/mL,灵敏度为(1.74±0.09)ng/mL。人体尿液样本的添加回收率为80.02%~102.69%,为后续开发供临床使用的诊断试剂奠定基础。N^(1),N^(12)-diacetylspermine is the diacetylated derivatives of spermine.Some studies show that the levels of N^(1),N^(12)-diacetylspermine in urine samples of many cancer patients including colorectal cancer,breast cancer,ovarian cancer,liver cancer,genitourinary cancer and non-small cell lung cancer are significantly higher than those of normal people.Based on the preparation of N^(1),N^(12)-diacetylspermine polyclonal antibody,a chemiluminescence immunoassay for the detection of N^(1),N^(12)-diacetylspermine in human urine samples was developed by optimizing the parameters of coating agent,blocking solution and antibody dilution ratio.The limit of detection in this method is(0.19±0.04)ng/mL,and the sensitivity is(1.74±0.09)ng/mL.The recovery rate of human urine samples is 80.02%to 102.69%.The method lays a foundation for the development of diagnostic reagents for clinical use.

关 键 词：N^(1) N^(12)-二乙酰精胺 化学发光免疫分析 肿瘤 

分 类 号：R730.43[医药卫生—肿瘤]