Long-term fundus fluorescence angiography and real-time diagnosis of retinal diseases in non-human primate-animal models  

作　　者：Miaomiao Tang Lu Zhang Bin Song Xiaoyuan Ji Chenyu Wang Houyu Wang Hua Xu Yuanyuan Su Yao He 

机构地区：[1]Laboratory of Nanoscale Biochemical Analysis,Institute of Functional Nano and Soft Materials(FUNSOM)and Collaborative Innovation Center of Suzhou Nano Science and Technology,Soochow University,Suzhou,215123,China [2]Department of Ophthalmology,Children’s Hospital of Soochow University,Soochow University,Suzhou,215123,China [3]Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation,Medical Research Center,Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University,Guangzhou,510120,China

出　　处：《Nano Research》2021年第11期3840-3847,共8页纳米研究（英文版）

基　　金：We appreciate financial support from National Natural Science Foundation of China (Nos. 21825402, 31400860, 21575096, and 21605109);Natural Science Foundation of Jiangsu Province of China (Nos. BK20191417 and BK20170061);the Program for Jiangsu Specially-Appointed Professors to Prof. Yao He, a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

摘　　要：Fluorescein angiography(FA)is a standard imaging modality for evaluating vascular abnormalities in retina-related diseases,which is recognized as the major cause of vision loss.Long-term and real-time fundus angiography is of great importance in preclinical research,nevertheless remaining big challenges up to present.In this study,we demonstrate that long-term fluorescence imaging of retinal vessels is enabled through a kind of fluorescent nanoagents,which is made of small-sized(hydrodynamic diameter:∼3 nm)silicon nanoparticles(SiNPs)featuring strong fluorescence,robust photostability,lengthened blood residency and negligible toxicity.In particular,the presented SiNPs-based nanoagents are capable of imaging retinal capillaries in∼10 min,which is around 10-fold longer than that(∼1 min)of fluorescein sodium(FS,known as the most widely used contrast agents for FA in clinic).Taking cynomolgus macaques as non-human primate-animal model,we further demonstrate the feasibility of real-time diagnosis of retinal diseases(e.g.,age-related macular degeneration(AMD))through dynamic monitoring of vascular dysfunction.

关 键 词：fluorescent silicon nanoparticles LONG-TERM real-time BIOIMAGING retinal diseases 

分 类 号：R774.1[医药卫生—眼科]