脯氨酸羟化酶3在乳腺癌中的表达及其对乳腺癌细胞恶性表型的影响  

Expression of Egl nine homolog 3 in breast cancer and its effect on malignant phenotype of breast cancer cells

作　　者：王杨 赵梓彤 宋咏梅 张柏林 WANG Yang;ZHAO Zitong;SONG Yongmei;ZHANG Bailin(Department of Breast Surgery,National Cancer CenterNational Clinical Research Center for CancerCancer Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,100021,China;State Key Laboratory of Molecular Oncology,National Cancer CenterNational Clinical Research Center for CancerCancer Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,100021,China)

机构地区：[1]国家癌症中心,国家肿瘤临床医学研究中心,中国医学科学院北京协和医学院肿瘤医院乳腺外科,北京100021 [2]国家癌症中心,国家肿瘤临床医学研究中心,中国医学科学院北京协和医学院肿瘤医院分子肿瘤学国家重点实验室,北京100021

出　　处：《中国医药导报》2021年第28期8-12,34,共6页China Medical Herald

基　　金：国家自然科学基金资助项目(81872398)。

摘　　要：目的研究脯氨酸羟化酶3(EGLN3)在乳腺癌中的表达情况及功能。方法使用GEPIA网站,分析EGLN3在TCGA和GTEx数据库中1085例乳腺癌组织和291例正常组织样本中的表达。选取2010年1月至2017年12月中国医学科学院北京协和医学院肿瘤医院诊治的52例乳腺癌患者的癌组织和癌旁组织,采用real-time PCR方法检测EGLN3 mRNA的表达水平。Western blot法检测EGLN3蛋白在正常乳腺上皮细胞(MCF-10A)和11株乳腺癌细胞(ZR-75-1、SK-BR-3、MDA-MB-453、BT474、MCF-7、T47D、MDA-MB-231、CAL-51、HS578T、MDA-MB-468、HCC1937)中的表达情况。采用xCELLigence RTCA MP系统、克隆形成实验和Transwell实验分别检测转染siRNA敲降EGLN3后对乳腺癌细胞增殖、克隆形成、侵袭和迁移能力的影响。结果乳腺癌组织EGLN3 mRNA表达高于正常组织,差异有统计学意义(P<0.05)。乳腺癌组织EGLN3 mRNA表达高于癌旁组织,差异有高度统计学意义(P<0.01)。EGLN3蛋白在乳腺癌细胞MDA-MB-231、CAL-51、MDA-MB-468和HCC1937中高表达,而在正常乳腺上皮细胞及其他乳腺癌细胞中不表达。转染siRNA敲降EGLN3后,乳腺癌细胞的增殖、克隆形成能力、侵袭和迁移能力均降低。结论EGLN3表达可能与乳腺癌的发生发展有关,下调EGLN3可抑制乳腺癌细胞的恶性表型,但其机制需进一步研究。Objective To investigate the expression and function of the Egl nine homolog 3(EGLN3)in breast cancer.Methods The expression of EGLN3 in 1085 cases of breast cancer tissues and 291 cases of normal tissues from the TCGA and GTEx databases was analyzed by using GEPIA website.A total of 52 cases of breast cancer tissues and adjacent tissues were collected in Cancer Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College from January 2010 to December 2017.The EGLN3 mRNA expression in breast cancer tissues was detected by real-time PCR.EGLN3 protein expression in normal breast epithelial cell(MCF-10A)and 11 cell lines of breast cancer(ZR-75-1,SK-BR-3,MDA-MB-453,BT474,MCF-7,T47D,MDA-MB-231,CAL-51,HS578T,MDA-MB-468,HCC1937)were detected by Western blot.The effect of transfection siRNA knockdown EGLN3 on proliferation,clonal formation,invasion and migration of breast cancer cells were detected by xCELLigence RTCA MP system,clonal formation assay and Transwell assay,respectively.Results The expression of EGLN3 mRNA in breast cancer tissues was higher than that in normal tissues,and the difference was statistically significant(P<0.05).The expression of EGLN3 mRNA in breast cancer tissues was higher than that in adjacent tissues,and the difference was highly statistically significant(P<0.01).EGLN3 protein was highly expressed in breast cancer cells MDA-MB-231,CAL-51,MDA-MB-468,and HCC1937,but not in normal breast epithelial cells and other breast cancer cells.After transfection with siRNA knockdown EGLN3,the proliferation,clonal formation,invasion and migration of breast cancer cells were decreased.Conclusion EGLN3 expression may be related to the occurrence and development of breast cancer,and down-regulation of EGLN3 can inhibit the malignant phenotype of breast cancer cells,but its mechanism needs further study.

关 键 词：脯氨酸羟化酶3 乳腺癌 增殖 侵袭 迁移 

分 类 号：R737.9[医药卫生—肿瘤]