高危型HPV-E6/E7mRNA检测在宫颈病变筛查中的临床价值  

Clinical Value of High-risk HPV-E6/E7 mRNA Detection in Cervical Lesions Screening

作　　者：姜洪苗[1] JIANG Hong-miao(Department of Obstetrics and Gynecology,the Second Affiliated Hospital of Nanjing Medical University,Nanjing 210000,Jiangsu,China)

机构地区：[1]南京医科大学第二附属医院妇产科,江苏南京210000

出　　处：《医学信息》2021年第22期163-165,共3页Journal of Medical Information

摘　　要：目的分析高危型人乳头瘤病毒(HR-HPV)-E6/E7mRNA检测在宫颈病变筛查中的临床价值。方法选取2020年2月-2021年2月南京医科大学第二附属医院共80例就诊患者作为研究对象,所有患者均接受阴道镜活检、HPV分型检测以及HR-HPV-E6/E7 mRNA检测,并以阴道镜活检病理结果作为金标准,分析HPV分型检测以及HR-HPV-E6/E7 mRNA检测结果,及HR-HPV-E6/E7 mRNA检测与宫颈病变病理分级的相关性。结果HPV检测显示阳性44例,占比55.00%;阴性36例,占比45.00%;阳性中正常或炎症占比低于阴性,CIN1、CIN2以及CIN3占比均高于阴性,差异有统计学意义(P<0.05);阳性与阴性ICC占比比较,差异无统计学意义(P>0.05)。HR-HPV-E6/E7 mRNA检测显示阳性42例,占比52.50%;阴性38例,占比47.50%;阳性正常或炎症占比低于阴性,CIN1、CIN2以及CIN3均高于阴性,差异有统计学意义(P<0.05);阳性与阴性ICC占比比较,差异无统计学意义(P>0.05)。HPV阳性率与HR-HPV-E6/E7 mRNA阳性率比较,差异无统计学意义(P>0.05)。Spearman相关性分析显示,HR-HPV-E6/E7 mRNA阳性与CIN1、CIN2以及CIN3呈正相关(r=0.670、0.510、0.600,P<0.05)。结论HR-HPV-E6/E7 mRNA检测阳性与宫颈病变病理结果密切相关,有利于临床医师判断患者病变的严重程度,且对患者预后具有一定的指导意义。Objective To analyze the clinical value of high-risk human papillomavirus(HR-HPV)-E6/E7 mRNA in the screening of cervical lesions.Methods A total of 80 patients in the Second Affiliated Hospital of Nanjing Medical University from February 2020 to February 2021 were selected as the research objects.All patients underwent colposcopy biopsy,HPV genotyping and HR-HPV-E6/E7 mRNA detection.The pathological results of colposcopy biopsy were used as the gold standard to analyze the results of HPV genotyping and HR-HPV-E6/E7 mRNA detection,and the correlation between HR-HPV-E6/E7 mRNA detection and pathological grading of cervical lesions.Results HPV test showed positive in 44 cases,accounting for 55.00%;negative 36 cases,accounting for 45.00%;the proportion of normal or inflammation in positive was lower than that in negative,and the proportion of CIN1,CIN2 and CIN3 was higher than that in negative,the difference was statistically significant(P<0.05).HR-HPV-E6/E7 mRNA detection showed 42 positive cases,accounting for 52.50%;negative 38 cases,accounting for 47.50%;the proportion of positive normal or inflammation was lower than negative,CIN1,CIN2 and CIN3 were higher than negative,the difference was statistically significant(P<0.05).There was no significant difference between the positive rates of HPV and HR-HPV-E6/E7 mRNA(P>0.05).Spearman correlation analysis showed that HR-HPV-E6/E7 mRNA positive was positively correlated with CIN1,CIN2 and CIN3(=0.670,0.510,0.600,<0.05).Conclusion The positive detection of HR-HPV-E6/E7 mRNA is closely related to the pathological results of cervical lesions,which is helpful for clinicians to judge the severity of patients',lesions,and has certain guiding significance for the prognosis of patients.

关 键 词：高危型人乳头瘤病毒 MRNA检测 宫颈病变 

分 类 号：R737.33[医药卫生—肿瘤]