一株野生血红小菇的分离鉴定及液体培养基配方筛选  

Isolation and Identification of a Wild Mycena haematopus sanguineus and Screening of Its Liquid Medium

作　　者：郭兴 高智涛 范冬茹 张德岩 刘继云 高云虹 GUO Xing;GAO Zhi-tao;FAN Dong-ru(Yichun Branch of Heilongjiang Academy of Forestry,Yichun,Heilongjiang 153000)

机构地区：[1]黑龙江省林业科学院伊春分院,黑龙江伊春153000

出　　处：《安徽农业科学》2021年第22期65-69,共5页Journal of Anhui Agricultural Sciences

基　　金：伊春市科技计划项目(G2018-7);黑龙江省自然科学基金项目(LH2021C083)。

摘　　要：血红小菇(Mycena haematopus(Pers.)P.Kumm.)是珍稀药用真菌,具有多种功能作用。液体菌种是血红小菇产业迈入标准化、规模化、工厂化的必由之路,而碳源、氮源、无机盐、维生素对血红小菇液体培养扩繁菌丝体具有重要影响。通过单因素试验和正交试验相结合,对菌丝含量进行测定,了解菌丝生长情况和各种参数之间的关系。结果显示,血红小菇液体最佳培养基配方为玉米粉40 g/L、蛋白胨4 g/L、KH_(2)PO_(4)1 g/L、MgSO_(4)0.7 g/L、NaCl 0.2 g/L、V_(B1)0.001%、pH 6.5。Mycena haematopus(Pers.)P.Kumm is a rare medicinal fungus with multiple functions.Liquid strain is the only way for the industry of Pleurotus sanguineus to be standardized,large-scale and industrialized.Carbon source,nitrogen source,inorganic salt and vitamin have an important influence on the liquid culture and propagation of mycelium of Pleurotus sanguineus.The content of hyphae was determined by combining single factor test and orthogonal test to understand the growth of mycelium and the relationship between the mycelium and various parameters.The results showed that the best liquid medium formula of Pleurotus eryngii was corn flour 40 g/L,peptone 4 g/L,KH_(2)PO_(4)1 g/L,MgSO_(4)7 g/L,NaCl 0.2 g/L,V_(B1)0.001%,pH 6.5.

关 键 词：血红小菇 液体培养基 发酵 

分 类 号：S646[农业科学—蔬菜学]