纯钛表面复合胶原蛋白凝胶涂层改性及其生物学性能初探  

Modification of collagen gel coating on pure titanium surface and its biological properties:a preliminary study

作　　者：张文思 朱文卿 邱憬[1,2,3] ZHANG Wesi;ZHU Wenqing;QIU Jing(Department of Oral Implantology,the Affiliated Stomatological Hospital of Nanjing Medical University,Nanjing 210029,China;Jiangsu Province Key Laboratory of Oral Diseases,the Affiliated Stomatological Hospital of Nanjing Medical University,Nanjing 210029,China;Jiangsu Province Engineering Research Center of Stomatological Translational Medicine,the Affiliated Stomatological Hospital of Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学附属口腔医院种植科,江苏南京210029 [2]南京医科大学附属口腔医院江苏省口腔疾病研究重点实验室,江苏南京210029 [3]南京医科大学附属口腔医院江苏省口腔转化医学工程研究中心,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2021年第9期1329-1335,共7页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金面上项目(81870799);江苏省重点研发计划(社会发展)项目(BE2019728);南京医科大学-苏盐智创新研发基金项目(NMU-SY201806);江苏高校优势学科建设工程资助项目(2018-87)。

摘　　要：目的:在纯钛表面制备生物胶原蛋白涂层,并探究其对成骨细胞行为的影响。方法:将Ⅰ型胶原蛋白制成凝胶,并附着于纯钛试件表面形成生物胶原蛋白涂层。以光滑钛表面为对照组,胶原蛋白涂层改性钛表面为实验组。采用扫描电镜观察试件表面微形貌,X射线光电子能谱(X-ray photoelectron spectroscopy,XPS)分析试件表面元素组成,红外光谱测试仪(fouriertransform infrared spectrometer,FT-IR)检测试件表面基团,接触角仪检测试件表面亲水性。体外培养MC3T3-E1成骨细胞,通过激光共聚焦显微镜、CCK-8、碱性磷酸酶(alkaline phosphatase,ALP)以及Western blot评价钛表面成骨细胞的黏附、增殖和分化能力。结果:扫描电镜观察发现实验组试件表面机械划痕变浅,XPS元素分析和FT-IR检测证实钛表面存在胶原蛋白涂层。接触角测量结果显示,实验组试件较对照组具有更好的表面亲水性。体外细胞实验结果显示,胶原蛋白涂层钛表面能促进成骨细胞的黏附和增殖活性,并上调ALP活性以及成骨相关蛋白Runx2、Osterix和OCN的表达。结论:纯钛表面复合胶原蛋白凝胶涂层改性能增强钛表面亲水性,促进成骨细胞的黏附、增殖和分化。Objective:This study aims to prepare a bio-collagen coating on pure titanium surface and investigate its effect on osteoblast behaviors.Methods:The type I collagen was made into gel and adhered to the surface of pure titanium specimens to form bio-collagen coating.The smooth titanium surface was used as the control group,and the collagen coated titanium surface was used as the experimental group.The surface microtopography of specimens was observed by scanning electron microscopy(SEM).The surface elemental compositions of specimens were analyzed via X-ray photoelectron spectroscopy(XPS).The groups on specimen surfaces were detected with fourier transform infrared spectrometer(FT-IR),and the surface hydrophilicity of specimens was tested by water contact angle meter.The adhesion,proliferation and differentiation of osteoblasts on titanium surfaces were evaluated by confocal laser scanning microscope(CLSM),CCK-8,alkaline phosphatase(ALP)detection and Western blot after the osteoblasts(MC3 T3-E1)were cultured in vitro.Results:SEM observation found the depth of mechanical scratches decreased on the surface of experimental specimens.XPS elemental analysis and FT-IR detection confirmed the presence of a collagen coating on titanium surface.Contact angle test results showed that the experimental group had better surface hydrophilicity than the control group.The results of in vitro study exhibited that collagen coated titanium surface could promote the adhesion and proliferation activity of osteoblasts and up-regulate ALP activity as well as the expressions of osteogenic-related proteins including Runx2,osterix and OCN.Conclusion:The modification of collagen gel coating on pure titanium surface can enhance its hydrophilicity and promote adhesion,proliferation and differentiation of osteoblasts.

关 键 词：胶原蛋白凝胶涂层 钛表面 成骨细胞行为 

分 类 号：R783.1[医药卫生—口腔医学]