重性抑郁症患者血液N-甲基-D-天冬氨酸受体NR1亚基mRNA水平及其与临床特征的关系  

mRNA level of N-methyl-D-aspartic acid receptor NR1 subunit in patients with major depressive disorder and its relationship with clinical features

作　　者：廖继武[1] 马萍[2] 杨海华 潘集阳[1] Liao Jiwu;Ma Ping;Yang Haihua;Pan Jiyang(Department of Psychiatry,the First Affiliated Hospital of Jinan University,Guangzhou 510632,China)

机构地区：[1]暨南大学附属第一医院精神医学科,广州510632 [2]暨南大学附属第一医院睡眠医学中心,广州510632 [3]暨南大学附属第一医院中心注射室,广州510632

出　　处：《中华医学杂志》2021年第39期3227-3231,共5页National Medical Journal of China

基　　金：广东省医院协会精准与舒适化医疗临床科研项目(2020-8)。

摘　　要：目的探讨重性抑郁症(MDD)患者谷氨酸能神经递质与神经传递功能紊乱特点及其与临床特征的关系。方法选取2019年8月至2020年8月在暨南大学附属第一医院就诊的MDD患者52例,社区招募健康对照51名,以汉密尔顿抑郁量表(HAMD-17)、汉密尔顿焦虑量表(HAMA)评估被试抑郁、焦虑症状,以酶联免疫吸附法测定血清谷氨酸水平,以逆转录聚合酶链反应检测NMDA受体NR1亚基mRNA水平。结果 MDD组与健康对照组间性别、年龄差异无统计学意义(P>0.05);MDD组血清谷氨酸水平[(35±6)mg/L 比(29±6)mg/L,P=0.021]及血液NR1亚基mRNA水平(1.5±0.8比0.8±0.6,P=0.001)高于健康对照组。MDD组血清谷氨酸水平与抑郁核心症状分呈正相关(r=0.52,P=0.028),NR1亚基mRNA水平与总病程呈正相关(r=0.42,P=0.024)。结论 MDD可能存在谷氨酸能神经递质水平紊乱及NR1亚基表达异常。Objective To investigate the characteristics of glutamatergic neurotransmitter and neurotransmission dysfunction in patients with major depressive disorder(MDD)and its relationship with clinical characteristics.Methods Fifty-two patients with MDD and 51 healthy controls were selected.Hamilton Depression scale-17(HAMD-17)and Hamilton Anxiety Scale(HAMA)were used to evaluate the symptoms of depression and anxiety.The serum glutamate level was measured by enzyme-linked immunosorbent assay(ELISA).Reverse transcription polymerase chain reaction(RT-PCR)was used to detect the mRNA level of NR1 subunit of NMDA receptor.Results There was no significant difference in gender and age between MDD group and healthy control group.Compared with the control group,the level of serum glutamate[(35±6)mg/L vs(29±6)mg/L,P=0.021]and mRNA level of NR1(1.5±0.8 vs 0.8±0.6,P=0.001)in MDD group were significantly higher.In MDD group,serum glutamate level was positively correlated with depression core symptom score(r=0.52,P=0.028),and mRNA level of NR1 subunit was positively correlated with the total course of disease(r=0.42,P=0.024).Conclusion MDD patients may have disorder of glutamatergic neurotransmitter and abnormal expression of NR1 subunit.

关 键 词：抑郁症 谷氨酸 N-甲基-D-天冬氨酸受体 

分 类 号：R749.4[医药卫生—神经病学与精神病学]