维生素K_(3)对14日龄北京鸭凝血时间和维生素K依赖蛋白的影响  

Effects of Vitamin K_(3) on Coagulation Time and Vitamin K-Dependent Proteins of 14-Day-Old Pekin Ducks

作　　者：申仲健 郝永胜 吴永保 张博 唐静[1] 侯水生[1] 谢明[1] SHEN Zhongjian;HAO Yongsheng;WU Yongbao;ZHANG Bo;TANG Jing;HOU Shuisheng;XIE Ming(State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100193

出　　处：《畜牧兽医学报》2021年第11期3304-3311,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家水禽产业技术体系建设专项(CARS-42-12);中国农业科学院科技创新工程重大科研任务(CAAS-ZDRW202104)。

摘　　要：旨在研究维生素K_(3)对1~14日龄北京鸭凝血时间和维生素K依赖蛋白的影响,筛选评价肉鸭维生素K营养状况的敏感指标,并得到育雏期北京鸭饲粮维生素K_(3)适宜添加量。试验采用单因子完全随机试验设计,设6个维生素K3添加水平(0、0.5、1、2、3和4 mg·kg^(-1)),通过在玉米-大豆分离蛋白型基础饲粮中添加6个不同添加水平亚硫酸氢钠甲萘醌配制试验饲粮。将180只1日龄健康雄性北京鸭随机分为6个处理组,每处理组5个重复,每重复6只鸭。试验期14 d。结果表明:不同添加水平维生素K_(3)对1~14日龄北京鸭平均日增重、平均日采食量和料重比等生长性能指标未产生显著影响(P>0.05)。0和0.5 mg·kg^(-1)维生素K_(3)添加组凝血酶原时间显著高于2、3、4 mg·kg^(-1)维生素K3添加组(P<0.05)。0 mg·kg^(-1)维生素K_(3)添加组血清凝血酶原前体蛋白水平显著高于0.5、1、2、3、4 mg·kg^(-1)维生素K3添加组(P<0.05);0 mg·kg^(-1)维生素K_(3)添加组血清羧化不全骨钙素水平显著高于2、3、4 mg·kg^(-1)维生素K3添加组(P<0.05)。以凝血酶原时间、血清凝血酶原前体蛋白和羧化不全骨钙素为评价指标,采用折线模型估算1~14日龄北京鸭维生素K3需要量分别为2.00、0.56和1.27 mg·kg^(-1)。综上,饲粮中添加维生素K_(3)可增强肉鸭凝血功能,降低血清凝血酶原前体蛋白和羧化不全骨钙素水平。在本试验基础饲粮条件下,以凝血酶原时间为评价指标,采用折线模型估测1~14日龄北京鸭维生素K3需要量为2.00 mg·kg^(-1)。This experiment was conducted to investigate the effects of vitamin K_(3) on coagulation time and vitamin K-dependent proteins of 14-day-old Pekin ducks.In order to screen sensitive indicators reflecting the nutritional status of vitamin K in meat ducks,and determine the vitamin K_(3) requirements of Pekin ducks.An one-factor completely randomize design with 6 vitamin K_(3) supplemental levels(0,0.5,1,2,3 and 4 mg·kg^(-1))was used in this experiment.The diets were formulated by adding 6 different levels of menadione sodium bisulfite to the corn-soybean isolate basal diets.A total of 180 one-day-old male Pekin ducks were randomly divided into 6 groups with 5 replicates each group and 6 ducks per replicate.The experiment lasted for 14 days.The results showed as follows:Different vitamin K3supplemental levels had no significant effect on the average daily gain,average daily feed intake and ratio of feed to gain of Pekin ducks from 1 to 14 days of age(P>0.05).The prothrombin time of the 0 and 0.5 mg·kg^(-1) vitamin K3 supplemental level groups were significantly higher than those of 2,3,and 4 mg·kg^(-1) vitamin K3 supplemental level groups(P<0.05).The level of serum PIVKA-Ⅱcontent of the 0 mg·kg^(-1) vitamin K3 supplemental level group was significantly higher than those of the 0.5,1,2,3,4 mg·kg^(-1) vitamin K3 supplemental level groups(P<0.05);The level of serum undercarboxylated osteocalcin content of the 0 mg·kg^(-1) vitamin K_(3) supplemental group was significantly higher than those of the 2,3,4 mg·kg^(-1) vitamin K_(3) supplemental level groups(P<0.05).Taking prothrombin time,serum PIVKA-Ⅱand undercarboxylated osteocalcin as evaluation indicators,vitamin K3 requirements of Pekin ducks from 1 to 14 days of age was determined as 2.00,0.56 and 1.27 mg·kg^(-1) by broken-line regression models.In conclusion,the supplemental of vitamin K_(3) to the diet could enhance coagulation performance,decrease the levels of serum vitamin K-dependent proteins such as the PIVKA-Ⅱand undercarboxylated osteocalcin.Und

关 键 词：鸭 维生素K 凝血功能 维生素K依赖蛋白 

分 类 号：S834.58[农业科学—畜牧学]