羧甲基化棉短绒止血纱布的制备及性能评价  

Preparation and properties of carboxymethylated cotton linters hemostatic gauze

作　　者：柳小军 徐玉茵 刘康博 周静 韩颖 熊玥 田源 Liu Xiaojun;Xu Yuyin;Liu Kangbo;Zhou Jing;Han Ying;Xiong Yue;Tian Yuan(Henan Medical Equipment Inspection Institute(Henan Medical Equipment Inspection and Testing Engineering Technology Research Center),Zhengzhou 450000,Henan Province,China;Nanyang Jiukang Medical Device Co.,Ltd.,Nanyang 473000,Henan Province,China)

机构地区：[1]河南省医疗器械检验所(河南省医疗器械检验检测工程技术研究中心),河南省郑州市450000 [2]南阳市久康医疗器械有限公司,河南省南阳市473000

出　　处：《中国组织工程研究》2022年第16期2473-2479,共7页Chinese Journal of Tissue Engineering Research

基　　金：河南省科技攻关项目(202102310467),项目负责人:柳小军。

摘　　要：背景:目前棉短绒及其改性制品在清理水或油污染方面受到广泛关注,然而将其用于止血纱布的制备尚未见报道。目的:以棉短绒纱布为原料改性制备一种羧甲基化棉短绒止血纱布,并对其进行表征。方法:以棉短绒纱布为原料,通过化学改性制备羧甲基化棉短绒止血纱布。采用红外光谱仪、扫描电镜和X射线衍射仪表征棉短绒纱布(原料组)、羧甲基化棉短绒止血纱布(实验组)与可吸收止血纱布(对照组)的形貌结构,通过测定吸水率、力学性能、凝胶化速率和溶解性等检测3种纱布的理化指标,通过检测全血凝血时间评价3种纱布的止血性能,通过体外细胞毒性实验评价实验组纱布的细胞相容性。结果与结论:(1)红外光谱显示,实验组纱布发生了羧甲基化反应,纤维结构中引入了亲水性羧甲基基团;X射线衍射结果显示,原料组的晶相较完整,实验组和对照组的晶相不完整,结晶较差;(2)扫描电镜显示,原料组纤维呈直线状且排列整齐紧密;实验组纤维编织方式未发生改变,但排列松散,纤维直径增加,表面粗糙度增加;对照组纤维具有独特的编织方式,纤维表面细长光滑且纹理清晰;(3)3组纱布的孔隙率比较差异无显著性意义(P>0.05);实验组的吸水率大于原料组(P<0.05),小于对照组(P<0.05);(4)实验组和对照组均在吸水后30 s内形成凝胶,并在1 h内完全溶解;(5)实验组的拉伸强度小于原料组(P<0.05),与对照组比较差异无显著性意义(P>0.05);实验组的断裂伸长率大于原料组(P<0.05),小于对照组(P<0.05);(6)实验组的全血凝固时间短于原料组(P<0.05),与对照组比较差异无显著性意义(P>0.05);实验组纱布具有良好的细胞相容性;(7)结果表明,羧甲基化棉短绒止血纱布具有良好的理化性能、止血性能和细胞相容性。BACKGROUND: At present, cotton lint and its modified products have been widely concerned in cleaning water or oil pollution, but the preparation of cotton lint for hemostatic gauze has not been reported. OBJECTIVE: To prepare a kind of carboxymethylated cotton linters hemostatic gauze by modifying cotton linters gauze and characterize it.METHODS: Carboxymethylated cotton linters cotton gauze was prepared by chemical modification. Infrared spectrometer, scanning electron microscope and X-ray diffractometer were used to characterize the morphology and structure of cotton linter gauze group(raw material), carboxymethylated cotton linter hemostatic gauze(experimental group) and absorbable hemostatic gauze(control group). The physical and chemical indexes were measured by water absorption, mechanical properties, gelation rate and solubility. The hemostatic performance was tested by measuring whole blood coagulation time. At last, the cytocompatibility of gauze in the experimental group was evaluated by cytotoxicity test in vitro. RESULTS AND CONCLUSION:(1) The result of infrared spectrum showed that carboxymethylation occurred in the gauze from experimental group, The hydrophilic carboxymethyl group was introduced into the fiber structure. X-ray diffraction results showed that compared with the raw material group, the crystal phase of the experimental and the control groups was incomplete with the crystallinity decreased.(2) Scanning electron microscope result showed that the fibers of the raw material group were straight and tightly arranged. In the experimental group, the fiber braiding mode was not changed, but the arrangement was loose, the fiber diameter was increased, and the surface roughness was increased. In the control group, the fiber had a unique weaving mode;the fiber surface was long and thin, smooth and the texture was clear.(3) There was no significant difference in porosity among the three groups(P > 0.05). The water absorption rate of the experimental group was higher than that of the raw material gr

关 键 词：材料 棉短绒纱布 羧甲基化 理化性能 形貌结构 止血性能 细胞相容性 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]