Carrageenan oligosaccharides and associated carrageenan-degrading bacteria induce intestinal inflammation in germ-free mice  

作　　者：Yeshi Yin Miaomiao Li Weizhong Gu Benhua Zeng Wei Liu Liying Zhu Xionge Pi Donald A.Primerano Hongwei D.Yu Hong Wei Guangli Yu Xin Wang 

机构地区：[1]State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Institute of Food Research,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China [2]Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology,Key Laboratory of Marine Drugs of Ministry of Education,Ocean University of China,Qingdao 266100,China [3]Department of Gastroenterology,Children's Hospital,Zhejiang University School of Medicine,Hangzhou 310057,China [4]Department of Laboratory Animal Science,College of Basic Medical Sciences,Third Military Medical University,Chongqing 400038,China [5]Department of Biomedical Sciences,Joan C.Edwards School of Medicine,Marshall University,Huntington,WV 25701,United States

出　　处：《Journal of Genetics and Genomics》2021年第9期815-824,共10页遗传学报（英文版）

基　　金：supported by National Natural Science Foundation of China(NSFC,31870106);supported by National Natural Science Foundation of China(NSFC,81991522);Key Research&Development of Zhejiang Province(2018C02048);State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts(2010DS0024-ZZ006);the National Science and Technology Major Project for Significant New Drug Development(2018ZX09735004);Taishan Scholar Climbing Project(TSPD20210304);supported by Distinguished Young Scholars of Hunan Natural Science Foundation(2020JJ2016);supported by NIGMS R44GM113545 and P20GM103434;supported by NIGMS WV-INBRE P20GM103434。

摘　　要：Carrageenans(CGNs)are widely used in foods and pharmaceuticals although their safety remains controversial.To investigate the effects of CGNs and CGN-degrading bacteria in the human colon,we screened for CGN degradation by human fecal microbiota,and for inflammatory response to CGNs and/or CGN-degrading bacteria in germ free mice.Thin-layer chromatography indicated that high molecular weight(MW)CGNs(!100 kDa)remained undegraded in the presence of human fecal microbiota,whereas low MW CGNs,i.e.,k-carrageenan oligosaccharides(KCO,~4.5 kDa)were degraded when exposed to seven of eight human fecal samples,although sulfate groups were not removed during degradation.Bacteroides xylanisolvens and Escherichia coli isolates from fecal samples apparently degraded KCO synergistically,with B.xylanisolvens serving as the primary degrader.Combined treatment of KCO with KCO-degrading bacteria led to greater pro-inflammatory effects in the colon and rectum of germ-free mice than either KCO or bacteria alone.Similarly,p-p38-,CD3-,and CD79a-positive immune cells were more abundant in combined treatment group mice than in either single treatment group.Our study shows that KCO-degrading bacteria and the low MW products of KCO can promote proinflammatory effects in mice,and represent two key markers for evaluating CGN safety in foods or medicines.

关 键 词：CARRAGEENANS Carrageenan oligosaccharides Oligosaccharide degrading bacteria Intestinal inflammatory Germ-free mouse 

分 类 号：R574[医药卫生—消化系统]