DAF rs150046210遗传变异与老年结直肠癌易感性关系研究  

Genetic variation of DAF rs150046210 affects the risk of colorectal cancer in the elderly

作　　者：金敏 仵红娇 付宁 张洪梅 宋琴琴 徐珊珊 纪珊珊 张雪梅 张志 JIN Min;SONG Qin-qin;XU Shan-shan;JI Shan-shan;ZHANG Zhi;WU Hong-jiao;FU Ning;ZHANG Hong-mei;ZHANG Xue-mei(Department of Oncology,Tangshan Workers Hospital Affiliated to North China University of Science and Technology,Tangshan 063000,China;College of Life Sciences,North China University of Science and Technology,Tangshan 063210,China)

机构地区：[1]华北理工大学附属唐山市工人医院肿瘤科,河北省唐山市063000 [2]华北理工大学生命科学学院,河北省唐山市063210

出　　处：《实用老年医学》2021年第11期1144-1148,共5页Practical Geriatrics

基　　金：河北省自然科学基金重点项目(H2017209233);河北省医学科学研究课题计划(20191562);唐山市科技创新团队培养计划(14130225B)。

摘　　要：目的探讨衰变加速因子(decay-accelerating factor,DAF)基因rs150046210多态性与结直肠癌发病的关系。方法收集来自唐山市工人医院的380例结肠癌病人和380例正常对照及450例直肠癌病人和450例正常对照的资料及血液样本,采用PCR凝胶电泳对DAF rs150046210进行基因分型。采用非条件Logistic回归分析DAF rs150046210变异与结直肠癌发病风险的关系。结果与DAF rs150046210 DD基因型携带者相比,Ⅱ基因型携带者结、直肠癌发病风险分别增加0.764倍(95%CI1.763~2.675,P=0.008)、0.789倍(95%CI1.227~2.608,P=0.003)。性别分层中,与DAF rs150046210 DD基因型携带者相比,携带Ⅱ基因型的女性患结肠癌风险增加1.015倍(95%CI1.085~3.742,P=0.026),携带Ⅱ基因型的男性患直肠癌风险增加1.041倍(95%CI1.290~3.228,P=0.002);年龄分层中,与DAF rs150046210 DD基因型携带者相比,携带Ⅱ基因型的低年龄组(≤60岁)患结肠癌风险增加(OR=2.335,95%CI1.273~4.356,P=0.006),携带DI、Ⅱ基因型的高年龄组患直肠癌风险也增加(OR=1.757,95%CI1.106~2.789,P=0.017;OR=2.313,95%CI1.334~4.012,P=0.003)。与DAF rs150046210 DD基因型携带者相比,携带Ⅱ基因型的不吸烟、不饮酒者患结肠癌风险分别增加0.791倍(95%CI1.106~2.900,P=0.018)、0.714倍(95%CI1.086~2.704,P=0.021),携带Ⅱ基因型的不吸烟、不饮酒者患直肠癌风险分别增加1.395倍(95%CI1.514~3.788,P<0.001)、0.923倍(95%CI1.221~3.030,P=0.005)。结论DAF rs150046210多态性可能增加老年人结直肠癌发病风险。Objective To explore the association of genetic variants of decay-accelerating factor(DAF)rs150046210 with the risk of colorectal cancer.Methods DAF rs150046210 was genotyped by Polymerase Chain Reaction in 380 patients with colon cancer and 380 normal controls,450 patients with rectal cancer and 450 normal controls,respectively.Logistic regression was used to investigate the association between rs150046210 and colorectal.Results Compared with DAF rs150046210 DD genotype carriers,Ⅱgenotype carriers had 0.764 times(95%CI 1.763-2.675,P=0.008)and 0.789 times(95%CI 1.227-2.608,P=0.003)higher risk of colon cancer and rectal cancer,respectively.When stratified by sex,women with genotypeⅡhad a 1.015-fold increased risk of colon cancer(95%CI 1.085-3.742,P=0.026),and men with genotypeⅡhad a 1.041-fold increased risk of rectal cancer(95%CI 1.290-3.228,P=0.002),comparing with DAF rs150046210 DD carriers.In the age stratification,this genetic variation affected the risk of colon cancer(OR=2.355,95%CI 1.273-4.356,P=0.006)in the low age group(≤60 years old)who carriedⅡgene,also affected the risk of rectal cancer(OR=1.757,95%CI1.106-2.789,P=0.017),(OR=2.313,95%CI1.334-4.012,P=0.003)in the high age group(>60 years old)with DI orⅡgenotype.Compared with DAF rs150046210 DD genotype carriers,Ⅱgenotype of not smoking,not drinkers had a 0.791(95%CI 1.106-2.900,P=0.018)and 0.714(95%CI 1.086-2.704,P=0.021)-fold increased risk of colon cancer incidence,typeⅡgene carriers who didn′t smoke or drink wine showed increased risk of rectal cancer(OR=2.395,95%CI1.514-3.788,OR=1.923,95%CI 1.221-3.030,respectively).Conclusions DAF rs150046210 polymorphism can increase the risk of colorectal cancer in the elderly patients.

关 键 词：衰变加速因子 补体调节蛋白 核苷酸多态性 结直肠癌 

分 类 号：R735.37[医药卫生—肿瘤]