分子生物学研究技术在玉簪属研究中的应用  

Application of Molecular Biology Technology to Hosta

作　　者：张燕[1] 李淑娟[1] 陈尘 Zhang Yan;Li Shujuan;Chen Chen(Shaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources,Institute of Botany of Shaanxi Province,Xi’an Botanical Garden of Shaanxi Province,Xi'an,710061)

机构地区：[1]陕西省西安植物园陕西省植物研究所陕西省植物资源保护与利用工程技术研究中心,西安710061

出　　处：《分子植物育种》2021年第20期6716-6722,共7页Molecular Plant Breeding

基　　金：西安市科技计划项目(20193024YF012NS012);陕西省自然科学基础研究计划项目(2021JM-598);陕西省重点研发计划农业攻关项目(2019NY-047)共同资助。

摘　　要：玉簪属(Hosta)植物是最受欢迎的园林植物之一,种类繁多,具有重要的生态、观赏、经济和文化价值。近年来不断发展完善的生物技术为玉簪的分子生物学研究提供了新的方法。本研究分别从玉簪遗传多样性分析和资源评价中分子标记技术(RAPD,SSR)的开发和应用;利用叶绿体基因组分析研究玉簪的系统发育;利用比较转录组学分析研究玉簪花色形成的机制;玉簪蛋白质组学研究;玉簪冷诱导基因(CBF1和DHN1)、抗氧化基因(SOD和CAT)、抗寒基因(HvGASA,HvFAD)及查尔酮合酶基因(HvCHS)和叶绿素生物合成相关基因(HrHEMA,HrPOR和HrCAO)的克隆和表达分析等方面综述了分子生物学研究技术在玉簪属研究中的应用,并进一步对玉簪分子生物学的研究方向进行了展望,以期为今后开展玉簪种质资源分子鉴定、遗传育种及开发利用等工作提供借鉴。Hosta is one of the most popular ornamental plants,and has important ecological,ornamental,economic and cultural values.In recent years,the continuous development of biotechnology has provided a new method for the research of Hosta.This study reviewed the following researches of Hosta molecular biology.The development and application of molecular marker techniques(RAPD,SSR) in genetic diversity analysis and resource evaluation of Hosta;The phylogenetic studies of Hosta depend on comparative analysis of chloroplast genomes;the mechanism of floral color formation of Hosta was studied by comparative transcriptome sequencing;the molecular mechanism of leaf variegation of Hosta was analyzed from the perspective of proteomics;cloning and expression analysis of Hosta cold-inducible genes(CBF1 and DHN1),antioxidant genes(SOD and CAT),cold-resistant genes(HvGASA,HvFAD),chalconesynthase genes(HvCHs) and chlorophyll biosynthesis genes(HrHEMA,HrPOR and HrCAO).In this study,the research direction of molecular biology of Hosta was prospected.This study was expected to provide references for the future research on molecular identification,genetic breeding,development and utilization of germplasm resources of Hosta.

关 键 词：玉簪属 分子生物学 叶绿体基因组 转录组学 蛋白质组学 

分 类 号：S682.19[农业科学—观赏园艺]