TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE_(2)分泌的影响  

Effect of Taurochenodeoxycholic Acid on PGE_(2)Excretion of Fibroblast-like Synoviocytes in Adjuvant Arthritis Rats with Stimulation of IL-1β

作　　者：姜雪莹 钱英红 赵俊利 张凯[1,2] 段艳 李培锋[1,2] 何秀玲[1,2] 毛伟 曹金山[1,2] 郭文瑞 JIANG Xue-ying;QIAN Ying-hong;ZHAO Jun-li;ZHANG Kai;DUAN Yan;LI Pei-feng;HE Xiu-ling;MAO Wei;CAO Jin-shan;GUO Wen-rui(Inner Mongolia Agricultural University,Hohhot 010018,China;Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease,Ministry of Agriculture and Rural Affairs,Hohhot 010018,China;Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)

机构地区：[1]内蒙古农业大学,内蒙古呼和浩特010018 [2]农业农村部动物疾病诊疗技术重点实验室,内蒙古呼和浩特010018 [3]内蒙古自治区农牧业科学院,内蒙古呼和浩特010031

出　　处：《畜牧与饲料科学》2021年第5期1-5,共5页Animal Husbandry and Feed Science

基　　金：内蒙古自治区自然科学基金项目(2016MS0348)。

摘　　要：[目的]进一步探讨牛磺鹅去氧胆酸(taurochenodeoxycholic acid,TCDCA)在抗炎免疫方面的潜在调节作用。[方法]以AA大鼠成纤维样滑膜细胞作为研究对象,采用ELISA方法检测TCDCA和IL-1β作用下AA大鼠成纤维样滑膜细胞上清液中PGE_(2)的含量,分析TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE_(2)分泌情况的影响。[结果]TCDCA能够对IL-1β刺激下AA大鼠纤维样滑膜细胞PGE_(2)的分泌产生下调作用(P<0.05)。[结论]TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE_(2)的分泌具有抑制作用,为TCDCA在兽医临床应用提供依据。[Objective]To further assess the potential anti-inflammatory and immunomodulatory roles of taurochenodeoxycholic acid(TCDCA).[Method]The fibroblast-like synoviocytes(FLS)of adjuvant arthritis(AA)rats were used as experimental research objects.The contents of prostaglandin E_(2)(PGE_(2))in supernatant of FLS co-incubated with TCDCA and IL-1βwere determined by ELISA to evaluate the effect of TCDCA on PGE_(2)excretion with stimulation of IL-1β.[Result]TCDCA significantly(P<0.05)reduced the PGE_(2)excretion level with stimulation of IL-1βin FLS of AA rats.[Conclusion]TCDCA has inhibitory effect on PGE_(2)excretion with stimulation of IL-1βin FLS of AA rats,which provides reference for application of TCDCA in veterinary clinic practice.

关 键 词：牛磺鹅去氧胆酸 成纤维样滑膜细胞 前列腺素E_(2) 

分 类 号：S853.74[农业科学—临床兽医学]