麻城黑山羊MC4R基因遗传多态性及生物信息学分析  

Polymorphism and bioinformatics analysis of MC4R gene in Macheng black goat

作　　者：郭帅 杨娟[2] 刘诗雨 杨丰利 熊琪[2] 汪招雄[1] GUO Shuai;YANG Juan;LIU Shi-yu;YANG Feng-li;XIONG Qi;WANG Zhao-xiong(School of Animal Science,Yangtze University,Jingzhou 434025,Hubei,China;Institute of Animal Husbandry and Veterinary Medicine,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)

机构地区：[1]长江大学动物科学学院,湖北荆州434025 [2]湖北省农科院畜牧兽医研究所,武汉430064

出　　处：《湖北农业科学》2021年第21期140-145,共6页Hubei Agricultural Sciences

基　　金：湖北省揭榜制科技项目(2020BED028);湖北省农业科技创新行动项目(2018skjcx05)。

摘　　要：黑素皮质素受体MC4R(melanoeortin receptor-4)是一种由下丘脑分泌的G蛋白偶联受体,其作用是调节动物采食量和体重。为了研究麻城黑山羊的遗传特异性,以MC4R为研究对象,检测其基因多态性,并进行生物信息学分析。结果表明,MC4R基因在麻城黑山羊中有8个突变位点,即C144T、T189A、A612G、C617A、C649G、C684A、T784A、C813A。根据理化性质分析可知,MC4R的氨基酸序列包含疏水区域,具有7个跨膜结构,编码蛋白的二级结构元件主要是α螺旋和无规则卷曲。不同物种间MC4R氨基酸序列比对发现,麻城黑山羊与绵羊的序列相似性为97.82%,与牛的序列相似性为94.50%,说明MC4R是保守蛋白,对动物的生长发育有重要作用。本研究通过测序获得8个MC4R基因的突变位点,并进行了生物信息学分析,为研究MC4R及其自然突变体影响山羊生长代谢的分子机制提供了理论依据。MC4R(melanocortin receptor-4)is a G protein coupled receptor secreted by hypothalamus,which regulates feed intake and body weight of animals.In order to study the genetic specificity of Macheng black goat,MC4R gene polymorphism was detected by sequencing,and biological characteristics of MC4R were predicted by bioinformatics analysis.The results showed that there were 8 mutation sites of MC4R gene in Macheng black goat,namely C144T,T189A,A612G,C617A,C649G,C684A,T784A and C813A.According to the analysis of physicochemical properties,the amino acid sequence of MC4R contains a hydrophobic region and has seven transmembrane structures.The secondary structural elements encoding the protein are mainly theαHelix and irregular curl.The amino acid sequence alignment of MC4R among different species showed that the sequence similarity between Macheng black goat and sheep was 97.82%,and that between Macheng black goat and cattle was 94.50%,indicating that MC4R was a conserved protein and plays an important role in animal growth and development.In this study,eight MC4R gene mutation sites were obtained by sequencing,and bioinformatics analysis was carried out,which provided a theoretical basis for studying the molecular mechanism of MC4R and its natural mutants affecting goat growth and metabolism.

关 键 词：麻城黑山羊 MC4R 突变 多态性 生物信息学 

分 类 号：S827[农业科学—畜牧学] Q879[农业科学—畜牧兽医]