两种血性胸腔积液的细胞蜡块制作方法对制片结果的影响  

Two methods of making cell wax blocks for bloody pleural effusion and their effect on the film-making results

作　　者：施丹飞 崔戈 李勇 SHI Danfei;CUI Ge;LI Yong(Department of Pathology,the First Affiliated Hospital of Huzhou University,Huzhou 313000,China)

机构地区：[1]湖州师范学院附属第一医院病理科,浙江湖州313000

出　　处：《温州医科大学学报》2021年第11期917-919,共3页Journal of Wenzhou Medical University

基　　金：浙江省教育厅科研项目(Y202043861);湖州市科技计划项目(2020GYB13)。

摘　　要：目的:比较传统细胞蜡块制作法(简称传统法)与改良细胞蜡块制作法(简称改良法)在血性胸腔积液中对苏木精-伊红(HE)制片及免疫组化制片结果的影响。方法:选取2018年1月至2021年1月湖州师范学院附属第一医院临床送检的渗出性血性胸腔积液患者样本55例,分别运用传统细胞蜡块制作法与改良细胞蜡块制作法进行制片,比较HE制片及免疫组化制片的优良率。结果:运用传统法的HE制片优良率为74.5%,免疫组化制片优良率为76.4%;运用改良法的HE制片优良率为94.6%,免疫组化制片优良率为92.7%。改良法的HE制片及免疫组化制片不良率均显著小于传统法(χ^(2)=8.419,P=0.004;χ^(2)=5.636,P=0.018)。结论:运用改良法制作血性胸腔积液样本细胞蜡块具有较好的制片效果。Objective:To compare the effect of traditional and modified cell wax methods on HE and immunohistochemistry in hemorrhagic pleural effusion.Methods:A total of 55 patients with exudative bloody pleural effusion admitted for clinical examination to the First Affiliated Hospital of Huzhou University from January 2018 to January 2021 were selected and prepared by the traditional and modified methods of cell wax block making respectively to compare the excellent rate of HE and immunohistochemical production.Results:The excellent rate of HE and immunohistochemistry by traditional method was 74.5%and 76.4%respectively,while it was 94.6%and 92.7%respectively by the modified method.The defect rate of HE and immunohistochemical in the modified method was significantly lower than that of the traditional method(χ^(2)=8.419,P=0.004;χ^(2)=5.636,P=0.018).Conclusion:The modified method has better effect on the preparation of cell wax blocks from hemorrhagic pleural effusion.

关 键 词：血性胸腔积液 细胞蜡块 优良率 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤] R446.8[医药卫生—临床医学]