磷脂酶A2对冠状动脉粥样硬化大鼠疾病严重程度的评估价值  

Value of Phospholipase A2 in Evaluation of the Severity of Coronary Atherosclerosis in Rats

作　　者：唐静怡[1] 段肖翠 范艳平[1] 刘立立 李静[1] 王健飞 史平 TANG Jing-yi;DUAN Xiao-cui;FAN Yan-ping;LIU Li-li;LI Jing;WANG Jian-fei;SHI Ping(Department of Cadre Wards,the 980 Hospital of PLA Joint Logistics Support Forces,Shijiazhuang 050082,China;Department of Cell Biology,School of Basic Medical Sciences,Hebei Medical University,Shijiazhuang 050017,China;Department of Clinical Medicine,Chengde Medical College,Chengde,Hebei 067000,China;the Second Retired Cadres'Sanitarium of Baoding City,Hebei Provincial Military Area Command,Baoding,Hebei 071000,China)

机构地区：[1]解放军联勤保障部队第九八〇(白求恩国际和平)医院干部病房,石家庄050082 [2]河北医科大学基础医学院细胞生物学教研室,石家庄050017 [3]承德医学院临床医疗系,河北承德067000 [4]河北省军区保定第二离职干部休养所,河北保定071000

出　　处：《解放军医药杂志》2021年第10期1-5,共5页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：河北省卫计委重点科技研究计划(20200231);河北省自然科学基金项目(H2019206535)。

摘　　要：目的探讨磷脂酶A2(Lp-PLA2)与冠状动脉粥样硬化大鼠疾病严重程度和稳定性的关系。方法SPF级雄性SD大鼠随机分为健康组、模型1组、模型2组。健康组给予普通饲料喂养并注射等量生理盐水;模型1组连续4 d腹腔注射维生素D320万U/(kg·d),并使用高脂饲料喂养7周;模型2组给予一次性腹腔注射维生素D380万U/kg,喂养方式、时间同模型1组。模型1组和模型2组分别在造模成功后使用阿托伐他汀灌胃4周;健康组大鼠给予等量生理盐水灌胃。记录各组大鼠体质量变化;测量各组大鼠主动脉粥样硬化斑块面积(PA)、血管管腔面积(LA),计算校正斑块面积(PA/LA);检测各组大鼠三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)及Lp-PLA2和超敏C反应蛋白(hs-CRP)水平。结果与健康组比较,模型1、2组大鼠喂养2、3周体质量增加(P<0.05)。模型1、2组大鼠造模后及治疗后血管LA低于健康组(P<0.05)。与造模后比较,模型1、2组治疗后血管LA增加,主动脉粥样硬化PA及PA/LA降低,但模型2组治疗后血管LA低于模型1组,主动脉粥样硬化PA及PA/LA高于模型1组(P<0.05)。与健康组比较,治疗后模型1、2组大鼠TG、TC、LDL-C、hs-CRP含量及Lp-PLA2活性均显著升高,且模型2组TG、TC、LDL-C、Lp-PLA2活性高于模型1组,但hs-CRP含量低于模型1组(P<0.05);与造模后比较,模型1、2组大鼠治疗后TG、TC、LDL-C、hs-CRP含量及Lp-PLA2活性均显著降低(P<0.05)。结论血清Lp-PLA2水平能反映冠状动脉粥样硬化疾病严重程度和稳定性,且随着冠状动脉粥样硬化病变严重程度和不稳定性增加而升高。Objective To investigate correlations between phospholipase A2(Lp-PLA2)with disease severity and stability of coronary atherosclerosis(CAS)in rats.Methods SPF male SD rats were randomly divided into healthy group,model group 1 and model group 2.The healthy group was fed with normal diet and injected with the same volume of physiological saline;model group 1 was given intraperitoneal injection of Vitamin D3[200,000 U/(kg)/d each time]for 4 d consecutively,and the rats were fed with high-fat diet for 7 weeks;model group 2 was given one-time intraperitoneal injection of Vitamin D3[800,000 U/(kg)],and the feeding method and time were the same with model group 1.Model group 1 and 2 were given Atorvastatin intragastrically for 4 weeks after successful modeling,while healthy rats were given the same volume of physiological saline by gavage.Body mass changes of rats were recorded in each group.Values of aortic atherosclerosis plaque area(PA)and vascular lumen area(LA)were measured,and the corrected plaque areas(PA/LA)were calculated,and levels of triglyceride(TG),total cholesterol(TC),low density lipoprotein-cholesterol(LDL-C),Lp-PLA2 and hypersensitive C-reactive protein(hs-CRP)were detected among all groups.Results Compared with those in healthy group,values of body weight of rats in model group 1 and 2 were significantly increased after feeding for 2 and 3 weeks(P<0.05).LA areas of rats in model group 1 and 2 after modeling and treatment were significantly lower than those in healthy group(P<0.05).Compared with those after modeling,LA areas were significantly increased,while values of aortic atherosclerosis PA and PA/LA were significantly decreased after treatment in model group 1 and 2;compared with those in model group 1,LA value was significantly lower,while values of PA area and PA/LA were significantly higher after treatment in model group 2(P<0.05).Compared with those in healthy group,levels of TG,TC,LDL-C and hs-CRP and the activity of Lp-PLA2 were significantly increased after treatment in model group 1 and 2;

关 键 词：冠状动脉疾病 磷脂酶A2 脂蛋白类 LDL 三酰甘油 C反应蛋白 大鼠 SPRAGUE-DAWLEY 

分 类 号：R-332[医药卫生] R541.4