嘌呤转运蛋白的缺失对枯草芽孢杆菌生产核黄素的影响  

Effect of purine transporter deletion on riboflavin production by Bacillus subtilis

作　　者：苏媛 刘川 谷振宇 夏苗苗[2] 钟成[1,3] 张大伟 SU Yuan;LIU Chuan;GU Zhenyu;XIA Miaomiao;ZHONG Cheng;ZHANG Dawei(College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China;Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China;State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science and Technology,Tianjin 300457,China;College of Biological and Pharmaceutical Sciences,China Three Gorges University,Yichang 443002,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]中国科学院天津工业生物技术研究所,天津300308 [3]天津科技大学,食品营养与安全国家重点实验室,天津300457 [4]三峡大学生物与制药学院,湖北宜昌443002

出　　处：《食品与发酵工业》2021年第22期16-23,共8页Food and Fermentation Industries

基　　金：国家重点研发计划(2020YFA0907800);天津市合成生物技术创新能力提升行动项目(TSBICIP-KJGG-004-03,TSBICIP-CXRC-004,TSBICIP-KJGG-011)。

摘　　要：该研究发现,发酵培养基中添加一定浓度的腺嘌呤/鸟嘌呤/黄嘌呤/次黄嘌呤会对枯草芽孢杆菌R的核黄素合成产生抑制。而在R中单独敲除7种嘌呤转运蛋白基因pbuG、nupG、pbuE、ywdJ、nupNOPQ、pbuX、pbuO后会分别减弱或增强嘌呤碱的抑制作用。将抑制作用变化所表征的转运蛋白功能与通过生长实验鉴定的转运蛋白的功能相结合,对这些转运蛋白的功能进行了判断。其中NupNOPQ、PbuX、PbuO具有腺嘌呤内运功能,PbuG和PbuO具有鸟嘌呤内运功能。在R中单独敲除nupNOPQ、pbuX和pbuG后核黄素产量分别提高了10.74%、5.84%、7.53%。而组合敲除三者后核黄素产量提高了19.14%,证明了嘌呤转运蛋白的敲除在提高枯草芽孢杆菌核黄素产量中具有一定作用。The addition of adenine/guanine/xanthine/hypoxanthine with a certain concentration could inhibit the riboflavin synthesis of Bacillus subtilis R.In strain R,deletion of one of the seven purine transporter genes(pbuG,nupG,pbuE,ywdJ,nupNOPQ,pbuX,and pbuO)could diminish or intensify the inhibition of purine bases on riboflavin production.The functions of the transporters were deduced from the combination of the riboflavin production of the deletion mutants treated with purine bases and the growth phenotypes of the deletion mutants.Among these transporters,NupNOPQ,PbuX,and PbuO could import adenine,while PbuG and PbuO could import guanine.After knocked out nupNOPQ,pbuX,and pbuG in strain R,the production of riboflavin increased by 10.74%,5.84%,and 7.53%,respectively.When all of the three genes were knocked out,the production of riboflavin increased by 19.14%.These results indicated that the deletion of purine transporters is effective in increasing riboflavin production.

关 键 词：嘌呤转运蛋白 嘌呤碱 枯草芽孢杆菌 核黄素 鸟苷三磷酸 

分 类 号：TQ924[轻工技术与工程—发酵工程]