两歧双歧杆菌FGSYC45M3缓解泻剂结肠及其作用机制分析  

Analysis of the effects on cathartic colon alleviation by Bifidobacterium bifidum FGSYC45M3 and its mechanism

作　　者：李鑫萍 王琳琳[1] 赵建新[1] 张灏[1] 陈卫[1] 王刚[1] LI Xinping;WANG Linlin;ZHAO Jianxin;ZHAO Hao;CHEN Wei;WANG Gang(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《食品与发酵工业》2021年第22期42-48,共7页Food and Fermentation Industries

基　　金：国家自然科学基金(81800469);江苏省自然科学基金(BK20180613)。

摘　　要：泻剂结肠是由于长期服用刺激类泻剂导致的肠神经受损,临床表现为顽固性便秘,目前缺乏有效的治疗手段。该研究通过灌胃番泻叶提取物建立小鼠泻剂结肠模型,分析两歧双歧杆菌FGSYC45M3缓解泻剂结肠小鼠便秘及修复其肠神经的效果,并探究其可能的作用机制。结果显示,两歧双歧杆菌FGSYC45M3能够显著缩短泻剂结肠小鼠的首粒黑便时间并提高粪便含水量。其作用机制可能与FGSYC45M3提高粪便中丁酸含量,恢复肠神经胶质细胞数量,提高胶质细胞源性神经营养因子(glial cell line-derived neurotrophic factor,GDNF)和乙酰胆碱酯酶(acetylcholinesterase,AChE)水平,增加结肠Cajal间质细胞(interstitial cells of Cajal,ICC)数量并降低水通道蛋白(aquaporins,AQPs)表达量相关。该研究提供了一种利用益生菌在缓解泻剂结肠患者便秘的基础上重建受损的肠神经系统,并建立正常的肠道稳态,以实现泻剂结肠缓解的思路,有望从根本上改善泻剂结肠。Cathartic colon is an intestinal nerve damage caused by long-term use of stimulating laxatives.Its clinical manifestation is intractable constipation.There is a lack of effective treatment for cathartic colon now.In this study,a mouse model of cathartic colon was established by gavage with senna leaf extract.The effects of constipation relief and colonic nerves repair by Bifidobacterium bifidum FGSYC45M3 were analyzed with this cathartic colon mouse model.Also,the mechanism of these effects was investigated.The results showed that B.bifidum FGSYC45M3 significantly shortened the time of the first black stool and increased the water content of feces.These beneficial effects may be related to the capabilities of FGSYC45M3 in increasing the content of butyric acid in feces,restoring the number of enteric glial cells,elevating the levels of glial cell line-derived neurotrophic factor(GDNF)and acetylcholinesterase(AChE),increasing the number of interstitial cells of Cajal(ICC)in the colon and decreasing the expression level of aquaporins(AQPs).This study provides a probiotic therapy for not only constipation alleviation,but also the intestinal nerves repair,which might alleviate the cathartic colon thoroughly.

关 键 词：泻剂结肠 双歧杆菌 肠神经 水通道蛋白 短链脂肪酸 

分 类 号：TS201.3[轻工技术与工程—食品科学]