不同护色处理对山药多糖的结构和免疫活性的影响  

Effects of different color-protection treatments on the structure and immune activity of yam polysaccharides

作　　者：严静 曾丽萍[1] 李俊健 黄达荣 黎攀 杜冰[1,2] YAN Jing;ZENG Liping;LI Junjian;HUANG Darong;LI Pan;DU Bing(College of Food,South China Agricultural University,Guangzhou 510642,China;Guangdong Laboratory for Lingnan Modern Agriculture,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]岭南现代农业科学与技术广东省实验室,广东广州510642

出　　处：《食品与发酵工业》2021年第22期199-207,共9页Food and Fermentation Industries

基　　金：国家现代农业产业技术体系资助(CARS-21);广东省重点领域研发计划项目(2020B020226008);广东省自然科学基金面上项目(2020A1515011268);广州城市职业学院科研团队项目(PK210201)。

摘　　要：为探究不同护色处理方式对山药多糖结构和免疫活性的影响。以经清水护色(water color-protection,CYH)、亚硫酸钠护色(sodium nitrite color-protection,CYN)、发酵液护色(fermentation color-protection,CYF)处理的山药为主要原料,提取并纯化不同护色处理的山药多糖及组分,通过高效凝胶渗透色谱等对多糖的结构进行初步鉴定,并建立RAW264.7细胞的免疫模型以探究其免疫刺激活性。结果表明,不同护色处理如清水护色、亚硫酸钠护色和发酵液护色处理对山药多糖的结构有显著影响,0.3 mol/L NaCl组分(CYH-3、CYN-3、CYF-3)的山药多糖结构差异最显著,分子质量、糖醛酸含量、单糖组成上均有显著差异。CYH-3、CYN-3、CYF-3的分子质量分别为4.06×10^(4)、2.57×10^(4)、2.99×10^(4)Da,糖醛酸含量分别为0.58%、3.99%、12.12%。在免疫活性方面,不同护色处理的山药多糖均能刺激RAW264.7细胞分泌TNF-α、IL-6、IL-1β、NO,但在特定浓度下,乳酸芽孢杆菌发酵护色的山药多糖的免疫活性明显高于亚硫酸钠护色。The aim of this study was to explore the effects of different color-protection treatments on the structure and immune activity of yam polysaccharides.Polysaccharides and components of yam treated with sodium nitrite,fermentation broth and water were extracted and purified.The structure of polysaccharides was preliminarily identified by high performance gel permeation chromatography(HPGPC),and the immune model of RAW264.7 cells was established to explore its immune stimulation activity.The results showed that the treatments of clear water(CYH),sodium sulfite(CYN)and fermentation broth(CYF)had significant effects on the structure of polysaccharide.The structure of yam polysaccharide with 0.3 mol/L NaCl component(CYH-3、CYN-3、CYF-3)was the most significant,and the molecular weight,glyoxylic acid content and monosaccharide composition were significantly different.The molecular weight of CYH-3、CYN-3、CYF-3 was 4.06×10^(4)、2.57×10^(4)、2.99×10^(4),and the content of glyoxylic acid was 0.58%、3.99%、12.12%,respectively.On the aspect of immune activity,yam polysaccharides treated with different color-protection could stimulate the secretion of RAW264.7 cells TNF-α、IL-6、IL-1β、NO,but at a specific concentration,the immune activity of yam polysaccharides fermented by Bacillus lactate is significantly higher than that of sodium nitrite.

关 键 词：山药多糖 护色处理 免疫活性 结构表征 

分 类 号：TS215[轻工技术与工程—粮食、油脂及植物蛋白工程]