当归挥发油提取及基于GC-MS的成分表征  

Extraction of Volatile Oil from Angelica Sinensis(Oliv)Diels and the Components Characterization Based on GC-MS

作　　者：冯媛 杨贵雅 张丹 王乾 陈璐 李子伊 郑玉光 张清清 FENG Yuan;YANG Guiya;ZHANG Dan;WANG Qian;CHEN Lu;LI Ziyi;ZHENG Yuguang;ZHANG Qingqing(College of Pharmacy,Hebei University of Chinese Medicine,Shijiazhuang 050200,China;Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province,Shijiazhuang 050091,China;Hebei Chemical&Pharmaceutical College,Shijiazhuang 050026,China)

机构地区：[1]河北中医学院药学院,石家庄050200 [2]河北省中药炮制技术创新中心,石家庄050091 [3]河北化工医药职业技术学院,石家庄050026

出　　处：《中国现代应用药学》2021年第19期2399-2404,共6页Chinese Journal of Modern Applied Pharmacy

基　　金：河北省中医药管理局科研计划项目(2019084);河北省自然科学基金项目(H2019423103);河北中医学院博士科研基金(BSZ2018004);河北中医学院优秀青年教师基础研究计划项目(YQ2018008)。

摘　　要：目的优选当归挥发性成分最佳提取工艺,并进行成分分析。方法采用水蒸气蒸馏法提取当归挥发油,以浸泡时间、提取时间、加水量为考察因素,以挥发油得率、Z-藁苯内酯相对百分含量、化合物个数及其综合评分为评价指标,通过正交设计试验结合信息熵法优选当归挥发油最佳提取工艺,同时利用GC-MS结合NIST数据库鉴定挥发油中的成分。结果优选的提取工艺为浸泡2 h、提取10 h、加水8倍量,在该条件下挥发油得率为0.38%,Z-藁苯内酯的相对百分含量为73.05%,共鉴定37个化合物;同时本实验基于GC-MS技术共鉴定55个化合物。结论优化后的水蒸气蒸馏法提取工艺重复性好、稳定可行,且可利用GC-MS分析当归挥发油中的化学成分,为当归的质量控制及药材资源的开发利用提供依据。OBJECTIVE To optimize the extraction process of volatile components of Angelica sinensis(Oliv)Diels,and to analyze the chemical components.METHODS The volatile oil from Angelica sinensis was extracted by steam distillation method,the investigate factors including soaking time,extracting time and the amount of water,and the yield of volatile oil,the relative percentage of Z-ligustilide,the number of compounds and the comprehensive score were used as the evaluation indexes.The optimal extraction process of volatile oil from Angelica sinensis was screened based on the orthogonal design test combined with information entropy method,meanwhile GC-MS combined with NIST database were used for the identification of the components in volatile oil.RESULTS The optimum extraction conditions were as follows:soaking for 2 h,extracting for 10 h and adding 8 times of water.Under these conditions,the extraction rate of essential oil was 0.38%,the relative content of Z-ligustilide was 73.05%,and 37 compounds were identified.And a total of 55 compounds were identified in this experiment based on GC-MS at the same time.CONCLUSION The optimal extraction process of steam distillation method is reproducible,stable and feasible,and the chemical constituents of the essential oil in Angelica sinensis can be characterized by GC-MS,which provide the basis for the quality control and the development and utilization of medicinal materials of Angelica sinensis.

关 键 词：当归 提取工艺 挥发油 Z-藁苯内酯 气质联用 

分 类 号：R284.1[医药卫生—中药学]