木犀草素与人免疫球蛋白G的作用机制研究  

Mechanism of interaction between luteolin and human immunoglobulin G

作　　者：李波[1,2] 秦小茹 AJABKhan 苗兰兰[4] 孙体健[1,2] 乔华[1,2] LI Bo;QIN Xiao-ru;AJAB Khan;MIAO Lan-lan;SUN Ti-jian;QIAO Hua(Shanxi Key Laboratory of Birth Defect and Cell Regeneration,Shanxi Medical University,Taiyuan 030001,China;Key Laboratory of Cellular Physiology(Shanxi Medical University),Ministry of Education,Taiyuan 030001,China;Department of Clinical Medicine,Fenyang College of Shanxi Medical University,Fenyang 032200,China;College of Pharmacy,Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学出生缺陷与细胞再生山西省重点实验室,山西太原030001 [2]山西医科大学细胞生理学教育部重点实验室,山西太原030001 [3]山西医科大学汾阳学院临床医学系,山西汾阳032200 [4]山西医科大学药学院,山西太原030001

出　　处：《化学研究与应用》2021年第11期2195-2202,共8页Chemical Research and Application

基　　金：山西省青年科技研究基金项目(201901D211339)资助;山西医科大学青年基金项目(02201507)资助;山西省重点学科建设经费资助。

摘　　要：采用紫外光谱、荧光光谱和圆二色谱以及分子对接技术,研究木犀草素(LUTE)与人免疫球蛋白G(hIgG)之间的相互作用。结果表明:LUTE对hIgG有较强的荧光猝灭作用,猝灭机理是形成复合物的静态猝灭,在形成LUTE/hIgG复合物时LUTE更靠近hIgG分子中的色氨酸残基。紫外光谱、三维荧光光谱及圆二色光谱实验结果发现,LUTE与hIgG结合时对蛋白分子中氨基酸残基的微环境影响较大,而对hIgG分子的骨架影响较小。分子模拟与荧光猝灭实验结果说明,二者结合是多种作用力综合作用的结果。The interaction between luteolin(LUTE)and human immunoglobulin G(hIgG)was studied by ultraviolet spectra(UV),fluorescence and circular dichroism(CD)spectra.The results suggested that LUTE had a strong ability to quench the hIgG fluorescence in a static mode.And LUTE was closer to the tryptophan residue in the hIgG molecule when LUTE and hIgG complexed.The results of UV,3D fluorescence and CD showed that LUTE had a great influence on the microenvironment of amino acid residues in hIgG protein molecules,but had little influence on the skeleton of hIgG.The results of molecular simulation and fluorescence quenching experiments showed that the combination of LUTE and hIgG was the result of a variety of forces.

关 键 词：木犀草素 免疫球蛋白 光谱法 分子对接 

分 类 号：O629[理学—有机化学]