华蟾素对人肝癌HepG2.2.15细胞体内外增殖的影响及可能的机制  

The effect of cinobufagin on proliferation of HepG2.2.15 cells in vitro and in vivo and its possible mechanism

作　　者：冯丽丽[1] 汪晓军 FENG Li-li;WANG Xiao-jun(Beijing You'an Hospital Affiliated to Capital Medical University,Beijing 10000,China)

机构地区：[1]首都医科大学附属北京佑安医院肝病四科,北京100000 [2]首都医科大学附属北京佑安医院中西医结合中心

出　　处：《中西医结合肝病杂志》2021年第11期1012-1016,共5页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：艾滋病和病毒性肝炎等重大传染病防治(No.2018ZX10303502-003)。

摘　　要：目的:探讨华蟾素抑制原发性肝癌的可能机制。方法:细胞学实验:应用华蟾素处理细胞,MTT法测定细胞增殖抑制率,流式细胞术检测细胞凋亡情况,PCR及Western blot检测细胞内p53的mRNA及蛋白的表达变化;动物学实验:构建裸鼠人肝癌移植瘤模型,华蟾素灌胃,检测裸鼠体重、肿瘤体积、瘤组织内p53蛋白的表达变化。结果:细胞学实验结果显示与对照组比较,华蟾素组HepG2.2.15细胞增殖率下降,凋亡增加,细胞中p53 mRNA及蛋白表达量明显增加,差异有统计学意义(P<0.05);动物学实验结果显示,华蟾素组荷瘤裸鼠的移植瘤体积缩小,瘤组织内p53蛋白的表达量增加,与对照组比较具有统计学差异(P<0.05)。结论:华蟾素抑制肝癌生长的机制可能与促进p53基因表达有关。Objective:To study the possible mechanism of cinobufagin in inhibiting hepatocellular carcinoma.Methods:In cytological experiments:Treat HepG2.2.15 cells with cinobufagin,then observe the proliferation of HepG2.2.15 cells by MTT method,use Flow cytometry to detect Cell apoptosis、PCR and Western blot to detect the expression of p53 gene and protein in cells.In zoology experiments:The model of hepatocellular carcinoma xenograft in nude mice was established,after intragastric administration of cinobufagin,the body weight,tumor volume and the expression of p53 protein were detected.Results:The results of cytological experiment showed that compared with the control group,the proliferation rate of HepG2.2.15 cells in cinobufagin group decreased,the apoptosis increased,and the expression of p53 mRNA and protein increased significantly(P<0.05);The results of animal experiments showed that the transplanted tumor volume of cinobufagin group decreased and the expression of p53 protein in tumor tissue increased,which was statistically different from that of the control group(P<0.05).Conclusion:The mechanism of cinobufagin inhibiting the growth of HCC may be related to the promotion of p53 gene expression.

关 键 词：原发性肝癌 华蟾素 P53基因 

分 类 号：R735.7[医药卫生—肿瘤]