不同酿酒葡萄品种对小新壳梭孢(N.parvum)的抗性评价及抗性相关酶研究  

Study on resistant evaluation of different wine grape varieties and resistance-related enzymes to N.parvum

作　　者：宋双 姜彩鸽 王国珍 张怡 SONG Shuang;JIANG Caige;WANG Guozhen;ZHANG Yi(Institute of Plant Protection,Ningxia Academy of Agro-Forestry Sciences/Ningxia Key Laboratory of Plant Disaeses and Pests Control,Yinchuan 750002,China)

机构地区：[1]宁夏农林科学院植物保护研究所/宁夏植物病虫害防治重点实验室,银川750002

出　　处：《中外葡萄与葡萄酒》2021年第6期49-53,共5页Sino-Overseas Grapevine & Wine

基　　金：宁夏农林科学院农业高质量发展和生态保护科技创新示范项目(NGSB-2021-4-04)宁夏回族自治区自然科学基金(2020AAC03312)财政部和农业农村部:国家现代农业产业技术体系资助(CARS-29-24)。

摘　　要：以贺兰山东麓13个酿酒葡萄品种为试材,通过室内刺伤绿枝接种方法,对比分析了这些品种的抗病性。其中高抗品种有‘维欧尼’‘长相思’‘黑比诺’‘威代尔’‘品丽珠’;低抗品种为‘霞多丽’。同时测定了接种小新壳梭孢(N.parvum)后过氧化物酶(POD)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性的变化。结果表明,供试品种对N.parvum的抗病性存在极显著差异。‘霞多丽’对N.parvum表现为低抗性,与其他品种差异显著;接种后潜育期1 d,病斑大小21.61 cm,菌斑扩增很大,产生的子实体潜育期短。接种N.parvum后,各酿酒葡萄叶片CAT、SOD和POD的活性均升高,高抗品种和低抗品种叶片抗性相关酶活性变化规律存在一定的差异。Thirteen main grapevine varieties from the eastern foot of Helan Mountain were used as the test materials.Wound inoculation method used on detached green branches was used to identify and evaluate the resistance of these varieties to N.parvum.At the same time,the changes of POD,SOD and CAT activities after inoculation with N.parvum were determined.The results showed that the resistance of thirteen wine grape varieties to N.parvum was significantly different.'Chardonnay'showed low resistance to N.parvum,which was significantly different from other varieties.The latent period was 1 d after inoculation,and the lesion size was 21.61 cm.After inoculation,not only the lesion was amplified greatly,but also the fruiting body was generated and the latent period was short.The activities of CAT,SOD and POD in leaves of grapevine varieties increased when N.parvum was inoculated,and there were some differences in the changes of leaf resistance-related enzyme activities between high and low resistant varieties.

关 键 词：酿酒葡萄 小新壳梭孢 抗病性 抗性相关酶 

分 类 号：S663.1[农业科学—果树学] S436.631.1[农业科学—园艺学]