高通量转录组RNA-seq研究成年牛下丘脑-垂体-卵巢  

Study on the Hypothalamus-Pituitary-Ovary of Adult Cattle by High-Throughput Transcriptome RNA-seq

作　　者：李剑波[1] 张佰忠[1] 何芳[1] 浣成[1] 罗阳 李晟[1] 蔡文杰[1] 伍佰鑫 李付强 易康乐[1] Li Jianbo;Zhang Baizhong;He Fang;Huan Cheng;Luo Yang;Li Sheng;Cai Wenjie;Wu Baixin;Li Fuqiang;Yi Kangle(Hunan Institute of Animal and Veterinary Science,Changsha,410130;Hunan Tianhua Industry Co.,Ltd.,Loudi,417100)

机构地区：[1]湖南省畜牧兽医研究所,长沙410130 [2]湖南天华实业有限公司,娄底417100

出　　处：《基因组学与应用生物学》2021年第4期1513-1521,共9页Genomics and Applied Biology

基　　金：湖南省重点研发计划项目(2016NK2171);国家重点研发计划项目(2018YFD050170);湖南省现代农业产业技术体系建设项目(湘财农指2019[97]);湖南创新型省份建设专项(2019NK3013-02)共同资助。

摘　　要：肉牛的性成熟受下丘脑-垂体-卵巢(HPO)轴的调节,但其具体作用机制尚不清楚。为了探讨HPO轴繁殖相关基因和调控机制,采用高通量转录组RNA-seq技术,研究了成年安格斯牛(30个月大)的下丘脑、垂体和卵巢。结果表明,HPO共有18 179个基因在GO (基因本体论)中注释,37 158个基因在KEGG (京都基因百科全书和基因组)中注释,36 645个基因在KOG (KEGG同源数据库)中注释。GO分析表明,HPO富集了8种生殖相关功能,主要是受精、单个组织生殖过程、子宫胚胎发育和雌激素反应。KEGG分析发现,HPO富集300条信号通路(SP),主要是癌症SP和磷脂酰肌醇3激酶蛋白激酶(PI3K-Akt)SP。与生殖有关的SPs共有16条,主要是Notch SP、神经营养素SP、哺乳动物雷帕霉素(mTOR) SP和血管内皮生长因子(VEG F) SP。为今后肉牛繁殖性能的提高、分子育种和同行参考提供了科学依据。The sexual maturity of beef cattle is regulated by the hypothalamus-pituitary-ovary(HPO) axis, but its specific mechanism is not clear. To explore the HPO-axis reproduction related genes and regulatory mechanisms,the hypothalamus, pituitary and ovary of adult Angus cattle(30 months of age) were studied by applying high-throughput transcriptome RNA-seq techniques. The results showed that the HPO had a total of 18 179 genes annotated in GO(Gene Ontology), 37 158 genes annotated in KEGG(Kyoto Encyclopedia of Genes and Genome), or 36 645 genes annotated in KOG(KEGG Ortholog database), respectively. GO analysis revealed that the HPO enriched eight kinds of reproduction related functions, mainly were fertilization, reproduction process of single tissue, embryonic development in uterus, and estrogen response. KEGG analysis found that the HPO enriched 300 signaling pathways(SPs), mainly cancer SP and phosphatidylinositol 3 kinase protein kinase(PI3 K-Akt) SP. There were a total of 16 SPs related to reproduction, mainly Notch SP, Neurotrophin SP, Mammalian target of rapamycin(mTOR) SP, and Vascular endothelial growth factor(VEGF) SP. It provided a scientific basis for future beef cattle improvement of reproductive performance, molecular breeding and peer reference.

关 键 词：牛 丘垂卵轴 RNA-SEQ 基因 信号通路 

分 类 号：S823[农业科学—畜牧学]