福建省甜玉米小斑病菌群体遗传结构和致病性分析  

Population Genetic Structure and Pathogenicity Analysis of Cochlibolus heterostrophus from Sweet Corn(Zea mays)in Fujian Province

作　　者：代玉立[1] 甘林[1] 廖蕾 滕振勇[2] 卢学松[1] 杨秀娟[1] DAI Yu-Li;GAN Lin;LIAO Lei;TENG Zhen-Yong;LU Xue-Song;YANG Xiu-Juan(Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests,Institute of Plant Protection,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China;Fujian Seed Station,Fuzhou 350001,China)

机构地区：[1]福建省农业科学院植物保护研究所/福建省作物有害生物监测与治理重点实验室,福州350013 [2]福建省种子总站,福州350001

出　　处：《农业生物技术学报》2021年第11期2198-2211,共14页Journal of Agricultural Biotechnology

基　　金：福建省农业科学院青年自由探索项目(AA2018-14);福建省农业科学院植物保护创新团队(CXTD2021027);福建省农业科学院“5511”协同创新工程(XTCXGC2021011);福建省属公益类科研院所专项(2018R1025-1)。

摘　　要：由异旋孢腔菌(Cochlibolus heterostrophus)引起的玉米小斑病(southern corn leaf blight,SCLB)是严重影响玉米(Zea mays)产量和品质的叶部真菌病害。为明确福建省甜玉米小斑病菌不同地理群体的群体遗传结构和致病性,本研究采用简单序列重复区间扩增多态性(inter-simple sequence repeat,ISSR)分子标记技术和致病性测定法分析福建省7个甜玉米小斑病菌群体(南平,宁德,福州,三明,莆田,龙岩和漳州)的遗传结构和致病性。利用优化的13条ISSR引物共扩增出198个位点,DNA多态性位点比例(percentage of polymorphic loci,P_(L))为100.0%,福州群体的DNA多态性水平最高(PL=73.7%),龙岩和漳州群体的DNA多态性水平最低(58.6%和56.6%);从福建省7个地理群体中共检测到126个多位点单倍型(multilocus haplotype),未检测到共享单倍型,南平群体的多位点单倍型多样性(multilocus haplotype diversity,HS)最丰富(H_(S)=0.323),龙岩群体的单倍型多样性较低(HS=0.157)。南平与宁德、莆田与三明以及龙岩与漳州两两群体间的遗传分化水平较低(Φ_(PT)<0.088),群体内基因交流频繁(Nm>5),而其他群体间的遗传分化水平较高。遗传相似系数为0.768时,福建省126个多位点单倍型菌株被划分为8个遗传类群,相同地理来源的菌株相对较集中。分子方差分析(analysis of molecular variance,AMOVA)显示群体内和群体间的遗传变异分别占总变异的81.9%和18.1%,表明福建省玉米小斑病菌群体遗传分化主要来源于群体内。主坐标(principal coordinates analysis,PCoA)和群体遗传结构分析表明福建省玉米小斑病菌分为2个遗传类群。致病性测定表明,7个地理来源病菌群体对4个抗感甜玉米品种均有较强的致病性,平均病情指数范围为36.42~59.23。病菌的致病力与菌株个体、地理来源以及玉米品种间存在极显著的差异(P<0.001),但是,其致病力与菌株×品种(P=0.999)以及地理来源×品种(P=0.361)互作因子Southern corn leaf blight(SCLB)caused by Cochlibolus heterostrophus is an important foliar fungal disease that significantly affects corn(Zea mays)yield and quality.To determine the population genetic structure and pathogenicity of C.heterostrophus populations from sweet corn in different geographical locations,inter-simple sequence repeat(ISSR)molecular markers and pathogenicity assays were used for analysis the population genetic structure and pathogenicity of 7 C.heterostrophus populations(Nanping,Ningde,Fuzhou,Sanming,Putian,Longyan,Zhangzhou)from sweet corn in Fujian province.A total of 198 loci were detected using optimized 13 ISSR primers,and the percentage of polymorphic DNA loci(P_(L))were100.0%,with the highest level of DNA polymorphism being detected from Fuzhou population(P_(L)=73.7%)and the lowest level of DNA polymorphism being detected from those in Longyan(LY)and Zhangzhou(ZZ)populations(58.6%and 56.6%).ISSR marker results indicated that a total of 126 multilocus haplotypes were detected among C.heterostrophus populations from the 7 geographical locations in Fujian province,with no shared multilocus haplotypes being detected from these populations.The most abundant multilocus haplotype diversity(H_(S))was detected from Nanping(NP)population(H;=0.323),whereas the lowest diversity was detected from those in LY(H_(S)=0.157).A low genetic differentiation was detected between C.heterostrophus populations from NP and Ningde,Putian and Sanming,and LY and ZZ(Φ;<0.088),with the frequent gene flow being detected between these populations(Nm>5).However,moderate to high genetic differentiation was detected among populations in the other geographical locations.One hundred and twenty-six multilocus haplotype isolates from sweet corn in Fujian province were divided into 8 genetic clusters at genetic similarity coefficient of 0.768,with different isolates originated from the same location clustering together.The analysis of molecular variance(AMOVA)of 7 C.heterostrophus populations indicated that approximately 8

关 键 词：玉米小斑病菌 多位点单倍型 遗传结构 致病力 甜玉米 ISSR分子标记 

分 类 号：S432.1[农业科学—植物病理学]