基于MoS_(2)量子点类过氧化物酶活性调控比色检测地中海贫血症治疗药物去铁酮  

Colorimetric detection of thalassaemia therapeutic drug deferiprone through tuning the peroxidase-like activity of MoS_(2) quantum dots

作　　者：谭娟 夏万强 付文升 王燚[1] Juan Tan;Wanqiang Xia;Wensheng Fu;Yi Wang(Engineering Research Center for Biotechnology of Active Substances(Ministry of Education),Chongqing Key Laboratory of Green Synthesis and Applications,College of Chemistry,Chongqing Normal University,Chongqing 401331,China)

机构地区：[1]活性物质生物技术教育部工程研究中心,绿色合成与应用重庆市重点实验室,重庆师范大学化学学院,重庆401331

出　　处：《中国科学：化学》2021年第11期1530-1538,共9页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:21775014);重庆市教委重点项目(编号:KJZD-K201900503)资助。

摘　　要：纳米酶催化活性的精准调控对其在传感和生物医学等领域的应用具有重要意义.本文以MoS_(2)量子点为研究对象,通过控制其在水溶液中的聚集或分散状态实现了类过氧化物酶活性的可控调节.研究发现,单分散的MoS_(2)量子点表现出十分微弱的类过氧化物酶活性,Fe^(3+)可诱导其发生聚集使粒径增大,导致类过氧化物酶活性显著增强;而地中海贫血症的治疗药物去铁酮能选择性与Fe^(3+)配位,从而抑制MoS2量子点的聚集行为和类酶活性增强,且这种抑制作用与去铁酮的浓度在一定范围内呈比例关系.基于该原理,本文构建了一种比色检测去铁酮的新方法并成功应用于药品中去铁酮的质量控制.借助智能手机的拍照和色度分析功能,本文还开发了利用智能手机作为检测器的便携式分析方法用于去铁酮含量的测定,该方法有望进一步扩展到床边检测与诊断.Precisely regulating the catalytic activity of nanozymes is of great significance for their applications in sensing,biomedicine,etc.In this work,the peroxidase-like activity of MoS_(2) quantum dots(QDs)is controlled by regulating their aggregation or disaggregation in aqueous solution.Specifically,monodispersed MoS_(2) QDs show very weak peroxidase-like activity.Fe^(3+)ions can induce significant aggregation of MoS_(2) QDs in aqueous solution and thus greatly enhance their activity.However,the drug deferiprone that commonly used for thalassaemia treatment can selectively bind with Fe^(3+)via complexation,thus inhibiting the aggregation of MoS_(2) QDs and their enhancement of enzyme-like activity.Based on this mechanism,a new colorimetric method for the detection of deferiprone is developed and further successfully applied to the quality control of drug.Moreover,with the aid of photographing and chroma analysis by a smartphone,a smartphone-based analytical method for deferiprone is also established,which is expected to be extended to point-of-care testing and diagnosis.

关 键 词：二硫化钼 纳米酶 地中海贫血 智能手机 比色分析 

分 类 号：O657.3[理学—分析化学] TQ460.72[理学—化学]