Evaluation of the immunomodulatory effects of C_(9-13)-CPs in macrophages  

作　　者：Zimeng Xue Jianbo Zhu Xia Wang Chunlei Yang Zhengwei Fu 

机构地区：[1]College of Biotechnology and Bioengineering,Zhejiang University of Technology,Hangzhou 310014,China

出　　处：《Acta Biochimica et Biophysica Sinica》2021年第9期1154-1165,共12页生物化学与生物物理学报（英文版）

基　　金：the grants from the Zhejiang Provincial Natural Science Foundation of China(No.LQ19B070006);the Program for Changjiang Scholars and Innovative Research Team in University(No.IRT17R97).

摘　　要：Short-chain chlorinated paraffins(SCCPs)have been listed as a new class of persistent organic pollutants by the Stockholm Convention.SCCPs exhibit carcinogenic-,endocrine-,and metabolism-disrupting effects.However,the knowledge of the immunomodulatory effects of SCCPs and their underlying mechanisms,especially in specific immune cells,remains limited.In addition to SCCPs,C_(9-13)-CPs have also been detected in humans.In this study,murine RAW264.7 macrophages were exposed to C_(9-13)-CPs at environmentally relevant concentrations to investigate whether or how C_(9-13)-CPs exhibit immunomodulatory effects.The results showed that the exposure of RAW264.7 cells to C_(9-13)-CPs increased cell viability,as assayed by MTT analysis at 490 nm,and also promoted cell proliferation,as indicated by EdU uptake assay,which was measured at excitation and emission wavelengths of 488 and 512 nm,respectively.In addition,exposure to C_(9-13)-CPs not only led to elevated ATP level and intracellular Ca2+level but also caused AMPK signaling activation and NF-κB signaling inhibition.Moreover,molecular docking showed that theβ2-AR receptor could bind to C_(9-13)-CPs.Taken together,these results suggest that the immune dysfunction of RAW264.7 cells caused by C_(9-13)-CPs is closely related to theβ2-AR/AMPK/NF-κB signaling axis.

关 键 词：SCCPs C_(9-13)-CPs RAW264.7 cells IMMUNOTOXICITY immunomodulatory cytokines 

分 类 号：R392[医药卫生—免疫学]