DA-JC4预处理对睡眠剥夺老龄大鼠认知功能的影响  被引量:1

Effects of DA-JC4 pretreatment on the cognitive function of sleep-deprived older rats

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作  者:黄松[1] 杜晓红[1] 徐国海[1] 罗明武 吴巧珍 Huang Song;Du Xiaohong;Xu Guohai;Luo Mingwu;Wu Qiaozhen(Department of Anesthesiology,the Second Affiliated Hospital of Nanchang University,Jiangxi Key Laboratory of Anesthesiology,Nanchang 330006,China)

机构地区:[1]南昌大学第二附属医院,江西省麻醉学重点实验室,330006

出  处:《国际麻醉学与复苏杂志》2021年第10期1042-1045,共4页International Journal of Anesthesiology and Resuscitation

基  金:江西省卫健委科技项目(20201067);江西省科技厅项目(2020ACBL206007)。

摘  要:目的评价DA-JC4预处理对睡眠剥夺老龄大鼠认知功能的影响。方法清洁级健康雄性SD大鼠36只,18~22月龄,体重500~600 g,采用随机数字表法分为3组(每组12只):对照组(C组)、睡眠剥夺组(S组)和DA-JC4组(D组)。S组和D组采用改良多平台水环境法建立老龄大鼠睡眠剥夺模型。D组每天10:00腹腔注射DA-JC425 nmol/kg(溶于1 ml生理盐水中),连续注射6 d,C组和S组于相同时间腹腔注射等量生理盐水。大鼠于睡眠剥夺结束后行Morris水迷宫实验,连续4 d,记录各组小鼠穿越平台次数及目标象限停留时间。于第5天水迷宫实验结束后处死大鼠取海马组织,采用ELISA法检测海马组织IL-1β、TNF-α含量,采用Western blot法检测海马细胞核NF-κB p65表达。结果与C组比较,S组和D组Morris水迷宫实验穿越平台次数减少,目标象限停留时间缩短,海马组织IL-1β、TNF-α含量增加,海马细胞核NF-κB p65表达上调(P<0.05);与S组比较,D组Morris水迷宫实验穿越平台次数增多,目标象限停留时间延长,海马组织IL-1β、TNF-α含量降低,NF-κB p65表达下调(P<0.05)。结论DA-JC4预处理可改善睡眠剥夺老龄大鼠的认知功能,其机制可能与抑制海马组织炎性反应有关。Objective To evaluate the effects of DA-JC4 pretreatment on the cognitive function of sleep-deprived elderly rats.Methods A total of 36 healthy male SD rats,aged 18-22 months and weighing 500-600 g,were divided into three groups according to the random number table method(n=12):a control group(group C),a sleep deprivation group(group S)and a DA-JC4 group(group D).The sleep deprivation model was established using elderly rats in group S and D in a modified multi-platform water environment.Rats in group D were intraperitoneally injected with 25 nmol/kg DA-JC4(dissolved in 1 ml normal saline)at 10:00 per day for consecutive six days.Those in groups C and S were intraperitoneally injected with the same volume of normal saline.After sleep deprivation,the rats were subjected to a Morris water maze experiment for four consecutive days,the times of crossing the platform and the time of staying in the target quadrant were recorded.At the end of the water maze experiment on day 5,the rats were sacrificed and the hippocampal tissues were taken.The levels of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)were detected by enzyme linked immunosorbent assay(ELISA),and the expression of nuclear protein nuclear factor kappa-B(NF-κB)p65 was measured by Western blot.Results Compared with group C,rats in groups S and D had less crossing times,shorter residence time in the target quadrant,increased levels of hippocampal IL-1βand TNF-α,and up-regulated expression of NF-κB p65(P<0.05).Compared with group S,group D presented increased crossing times,prolonged residence time in the target quadrant,decreased levels of IL-1βand TNF-αin the hippocampus,and down-regulated expression of NF-κB p65(P<0.05).Conclusions DA-JC4 pretreatment improves the cognitive function of sleep-deprived older rats,which may be associated with suppression of hippocampal inflammation.

关 键 词:睡眠剥夺 认知 炎症 

分 类 号:R749.1[医药卫生—神经病学与精神病学]

 

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