丁香水提物的抑菌效应及对兔标本保存效果的研究  

Effect of the antimicrobial activities of water extracts from clove on rabbit specimen preservation

作　　者：祖文轩 侯文瀚 王银燕 林娜 桂航 张倩楠 陈士文[1] ZU Wen-xuan;HOU Wen-han;WANG Yin-yan;LIN Na;GUI Hang;ZHANG Qian-nan;CHEN Shi-wen(Department of Anatomy,School of Basic Medicine,Bengbu Medical College,Bengbu Anhui 233030,China;School of Clinical Medicine,Bengbu Medical College,Bengbu Anhui 233030,China;School of Public Foundation,Bengbu Medical College,Bengbu Anhui 233030,China)

机构地区：[1]蚌埠医学院解剖教研室,安徽蚌埠233030 [2]蚌埠医学院临床医学院,安徽蚌埠233030 [3]蚌埠医学院公共基础学院,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2021年第11期1507-1509,共3页Journal of Bengbu Medical College

基　　金：蚌埠医学院自然科学基金重点项目(BYKY2019010ZD);国家级大学生创新创业训练计划项目(201910367022)。

摘　　要：目的:探讨丁香水提物的抑菌及组织保存作用。方法:用水提法提取丁香主要成分液体,以10%甲醛及蒸馏水作对照,测定3组液体对金黄色葡萄球菌及大肠杆菌的抑菌圈大小,和对兔皮组织保存效果,并采用二倍稀释法测定丁香水提抑菌液的最小抑菌浓度。结果:丁香水提液无刺激性气味,对金黄色葡萄球菌及大肠杆菌均有抑菌作用,最小抑菌浓度分别为125 mg/mL和62.5 mg/mL。作为保存液在12周内未有细菌检出。结论:丁香水提物具有一定的抑菌作用,在一定时间内有较好的组织保存作用。Objective:To study the effects of water extracts from clove on the antimicrobial activities and the tissue preservation.Methods:The extraction products of clove were obtained by water extraction,then the bacteriostatic ring size and tissue preservation effect were compared with 10%formalin group and distilled water group.The minimum inhibitory concentration was determined by double dilution method.Results:Water extracts from clove has no obvious irritating odor which has an inhibitory effect on both E.coli and Staphylococcus aureus.The minimum inhibitory concentrations were 125 mg/mL and 62.5 mg/mL,respectively.No bacteria in the water extracts from clove as preservative solution were detected in 12 weeks.Conclusions:The water extracts from clove has the certain bacteriostatic effect,which exerts a better preservation influence on the tissue specimen in a certain time.

关 键 词：标本保存 辛香料 丁香 保存液 

分 类 号：R322.34[医药卫生—人体解剖和组织胚胎学]