微小RNA-186在原发性无精子症病人外周血中的表达及诊断价值  

Expression and diagnostic value of microRNA-186 in peripheral blood of patients with idiopathic azoospermia

作　　者：佟凯军 张亮[1] 汤坤龙[2] 杨长海[2] TONG Kai-jun;ZHANG Liang;TANG Kun-long;YANG Chang-hai(Department of Urology,Chinese People′s Liberation Army No.983 Hospital,Tianjin 300000;Department of Urology,General Hospital of Tianjin Medical University,Tianjin 300052,China)

机构地区：[1]中国人民解放军第983医院泌尿外科,天津300000 [2]天津医科大学总医院泌尿外科,天津300052

出　　处：《蚌埠医学院学报》2021年第11期1572-1575,共4页Journal of Bengbu Medical College

摘　　要：目的:检测微小RNA-186(miR-186)在原发性无精子症(IA)病人外周血中的表达量,并探讨其对IA的诊断价值。方法:收集2015-2017年在泌尿外科就诊的IA病人(IA组)140例和正常生育的男性(对照组)140名的外周血标本。常规提取外周血DNA和RNA,采用多重PCR试验检测无精子症因子(AZF)在2组间的微缺失情况。采用实时荧光定量PCR试验检测miR-186在2组间的表达差异。分析外周血中miR-186的表达与AZF微缺失的相关性。采用ROC分析评价外周血中miR-186表达对IA的诊断价值。结果:2组年龄、体质量指数、睾酮水平及吸烟情况差异均无统计学意义(P>0.05)。IA组中8例病人存在AZF微缺失(P<0.05),微缺失率为5.71%(8/140),而对照组中未检测出AZF微缺失(P<0.05)。miR-186在IA组外周血中的表达量较对照组明显升高(P<0.01)。外周血中miR-186的表达与AZF微缺失无相关性(P>0.05)。miR-186对IA具有较高的诊断价值,ROC曲线下面积为0.84(95%CI:0.794~0.886),当miR-186表达量为0.52时为最佳诊断分界点,敏感度为71.86%,特异度为80.39%。结论:miR-186在IA病人外周血中高表达,但与AZF微缺失无相关性。外周血中高表达的miR-186可能作为IA的一种新的诊断标志物。Objective:To detect the expression levels of microRNA-186(miR-186)in the peripheral blood of patients with idiopathic azoospermia(IA),and investiagte its diagnostic value for IA.Methods:The peripheral blood samples from 140 IA patients from 2015 to 2017 in urology department and 140 normal fertile men were divided into the IA group and control group,respectively.The DNA and RNA in two groups were routinely extracted from peripheral blood,and the microdeletion of azoospermia factor(AZF)in two groups were detected using multiplex PCR.The expression levels of miR-186 in two groups were detected using quantitative real-time PCR.The correlation between miR-186 expression and AZF microdeletion in peripheral blood was analyzed.The diagnostic value of miR-186 for IA in peripheral blood was evaluated by ROC analysis.Results:The differences of the age,body mass index,testosterone level and smoking status were not statistically significant between two groups(P>0.05).Eight patients with AZF microdeletion in the IA group were found,and the microdeletion rate of which was 5.71%(8/140),while no AZF microdeletion was detected in the control group(P>0.05).The expression level of miR-186 in IA group was significantly higher than that in control group(P<0.01).The miR-186 expression in peripheral blood was not correlated with AZF microdeletion(P>0.05).The miR-186 had a higher diagnosis value for IA,and the area under the ROC curve was 0.84(95%CI:0.79 to 0.87).The expression level of miR-186 at 0.52 was the best diagnostic cut-off point,and the sensitivity and specificity were 71.86%and 80.39%,respectively.Conclusions:The expression level of miR-186 is high in the peripheral blood of IA patients,but its expression is not correlated with AZF microdeletion.High expression of miR-186 in peripheral blood may serve as a new diagnostic biomarker for IA.

关 键 词：原发性无精子症 微小RNA-186 微缺失 外周血 标志物 

分 类 号：R698.2[医药卫生—泌尿科学]