临床实验室浆膜腔积液细胞计数稳定性研究  

Study on the stability of cell count of serous fluid in clinical laboratory

作　　者：林潮庆 蒋伟燕[1] 李绵绵[1] 陈小剑[1] LIN Chao-qing;JIANG Wei-yan;LI Mian-mian;CHEN Xiao-jian(Department of Clinical Laboratory,the Second Affiliated Hospital of Wenzhou Medical University,Wenzhou,Zhejiang 325027,China)

机构地区：[1]温州医科大学附属第二医院医学检验中心,浙江温州325027

出　　处：《中国卫生检验杂志》2021年第20期2513-2516,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的探讨不同存储时间和温度对浆膜腔积液标本细胞计数稳定性的影响。方法选取25例浆膜腔积液标本,分别放置在4℃、室温25℃和37℃条件下,在即刻、1 h、2 h、4 h、8 h、12 h、24 h、36 h和48 h内应用Sysmex XE-5000血细胞分析仪体液模式进行检测。结果在体液标本中,白细胞计数在室温和4℃保存48 h内数值较为稳定,在37℃环境下,白细胞数值有减少的趋势,但其差异无统计学意义(P> 0.05);单个核细胞、多个核细胞分类48 h内结果无明显变化(P>0.05);红细胞计数在各温度下稳定。结论应用Sysmex XE-5000血液分析仪检测浆膜腔积液标本在无菌管内37℃、室温25℃及冷藏条件下,48 h内细胞计数结果稳定性均较高。Objective To investigate the effects of different storage time and storage temperatures on the stability of cell count results in serous effusion specimens. Methods Twenty-five serous effusion specimens were selected and placed at 4 ℃,room temperature( 25 ℃) and 37 ℃,respectively. Sysmex XE-5000 was applied immediately,at 1 h,2 h,4 h,8 h,12 h,24 h,and 48 h. The blood cell analyzer is tested in body fluid mode. Results In the humoral samples,the white blood cell count was stable after being stored at room temperature and 4 ℃ for 48 hours. At 37 ℃,the white blood cell counts decreased,but the difference was not statistically significant( P > 0. 05). There was no significant change in the results of single nuclear cells and multiple nuclear cells within 48 hours( P > 0. 05). The red blood cell count is very stable at all temperatures. Conclusion Sysmex XE-5000 blood analyzer was used to detect the blood cell count of serous effusion specimens at 37 ℃,room temperature and refrigerated conditions,and the stability of the results was higher within 48 hours.

关 键 词：浆膜腔积液 Sysmex XE-5000 存储时间 存储温度 

分 类 号：R73[医药卫生—肿瘤]