膀胱尿路上皮癌组织中γ-谷氨酰环化转移酶的表达及其临床意义  

The expression of GGCT in the bladder urothelial cell carcinoma and its clinical significance

作　　者：郭永顺[1] 吴育栋 马昊[2] 齐磊 张世栋 臧运江[1] Guo Yongshun;Wu Yudong;Ma Hao;Qi Lei;Zhang Shidong;Zang Yunjiang(Departmentof Urology,the People′s Hospital of Weifang,Weifang 261041,China;Department of Pathology,the People′s Hospital of Weifang,Weifang 261041,China)

机构地区：[1]山东省潍坊市人民医院泌尿外科,261041 [2]山东省潍坊市人民医院病理科,261041

出　　处：《中华肿瘤杂志》2021年第11期1183-1187,共5页Chinese Journal of Oncology

摘　　要：目的探讨膀胱尿路上皮癌组织中γ-谷氨酰环化转移酶(GGCT)的表达及其临床意义。方法收集2011年1月至2013年5月来自潍坊市人民医院的86例膀胱尿路上皮癌和10例癌旁正常膀胱黏膜组织,应用免疫组化染色方法检测GGCT蛋白的表达,并分析其与临床病理特征及预后的关系。结果 86例膀胱尿路上皮癌组织中GGCT表达阳性率为61.6%(53/86)。GGCT蛋白主要在肿瘤细胞的细胞质中表达,部分病例的细胞核中也有表达。GGCT蛋白的表达水平与膀胱尿路上皮癌的肿瘤最大径(P=0.025)、病理分级(P<0.001)和病理分期(P=0.020)有关。在非肌层浸润性膀胱尿路上皮癌中,T1期患者膀胱尿路上皮癌组织中GGCT蛋白的表达高于Ta期患者(P=0.034),GGCT阳性患者的无复发生存率低于GGCT阴性患者(P=0.029)。Cox多因素回归分析显示,肿瘤数量(OR=3.320,P=0.024)和病理分期(OR=5.029,P=0.009)是非肌层浸润性膀胱尿路上皮癌患者无复发生存的独立影响因素。结论 GGCT在膀胱尿路上皮癌组织中高表达,与膀胱尿路上皮癌的恶性生物学行为和进展有关。GGCT阳性的早期膀胱尿路上皮癌患者更容易发生复发。Objective To evaluate the expression and clinical significance ofγ-glutamylcyclotransferase(GGCT)in patients with bladder urothelial cell carcinoma.Methods Immunohistochemical staining for GGCT were performed on tissue sections of 86 patients with bladder urothelial cell carcinoma and 10 normal controls,and the correlations between GGCT and clinicopathological characteristics and the prognosis were analyzed.Results The positive rate of the expression of GGCT in 86 cases of bladder urothelial cell carcinoma was 61.6%(53/86).GGCT protein was located mainly in cancer cell cytoplasm,and it can be seen in the nucleus of the tumor cells in some cases.The level of GGCT expression was positively related to pathological classification(P<0.001),stage(P=0.020),and tumor size(P=0.025).Immunohistochemical semiquantitative analysis showed that the expression of GGCT in patients with T1 stage of non-muscle invasion bladder urothelial cell carcinoma was significantly higher than that with Ta stage(P=0.034).Kaplan-Meier analysis showed that the expression of GGCT was correlated with the recurrence-free survival in patients with non-muscle invasive bladder cancer,the recurrence-free survival rate was lower in the GGCT positive group(P=0.029).Multivariate COX regression analysis showed that the pathological stage(OR=5.029,P=0.009)and the number of tumors(OR=3.320,P=0.024)were the independent risk factors for recurrence-free survival in patients with early urothelial cell carcinoma of the bladder.Conclusions The expression of GGCT is significantly increased in bladder urothelial cell carcinoma and is related to the malignant biological behavior and progression of tumor.Patients with GGCT positive early bladder tumor are inclined to recur.

关 键 词：膀胱尿路上皮癌 γ-谷氨酰环化转移酶 免疫组织化学染色 预后 

分 类 号：R737.14[医药卫生—肿瘤]