光唇鱼对单色光偏好的性别差异及相关生理机制  

Sex differences in the preference for monochromatic light and the associated physiological mechanisms in a stream-dwelling fish, Acrossocheilus fasciatus

作　　者：颉志刚[1] 杨范利 XIE Zhigang;YANG Fanli(Zhejiang Provincial Key Laboratory of Biotechnology and Protective Utilization of Wild Animals,College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321004,China)

机构地区：[1]浙江师范大学化学与生命科学学院,浙江省野生动物生物技术与保护利用重点实验室,浙江金华321004

出　　处：《中国水产科学》2021年第10期1263-1271,共9页Journal of Fishery Sciences of China

基　　金：浙江省公益技术用研究计划国际合作项目(2017C34006)。

摘　　要：为探究溪流性鱼类光唇鱼(Acrossocheilus fasciatus)对光色的偏好是否存在性别差异,以及芳香化酶作为催化睾酮(T)向雌二醇(estradiol,E2)转化的限速酶在行为调节中的关键角色,本研究采用行为学方法研究了雌鱼和雄鱼对6种单色LED光源的选择偏好,并尝试利用药物抑制体内芳香化酶活力从而改变血浆性激素水平和鱼的行为表现。结果表明,雌鱼和雄鱼分别相对偏好红光(峰值为637 nm)和黄光(590 nm),两者均相对排斥蓝光(465 nm)、紫光(405 nm)以及含有蓝光波段的白光(447 nm),而对绿光(518 nm)的偏好不明显。摄食含有芳香化酶抑制剂来曲唑(letrozole,LZ)的实验饲料10d后,雌性和雄性光唇鱼脑和性腺组织内芳香化酶活力和血浆E2水平均显著下降,但血浆T水平无显著变化。值得注意的是, LZ也导致处理鱼对光色选择偏好的改变,雌鱼转为相对偏好蓝光,雄鱼转为相对偏好红光。然而,摄入含LZ+E2实验饲料后除芳香化酶活力降低外, T、E2两种性激素水平和光色偏好行为表现均未出现明显变化。因此推测光唇鱼对光色偏好的性别差异极可能与体内芳香化酶活力和雌二醇水平的性别差异有关,而与睾酮水平无明显关联。The aim of the present study was to investigate sex differences in light color preference and the key role of aromatase in the conversion of testosterone(T) to estradiol(E2) in the regulation of this preference in a stream-dwelling fish, Acrossocheilus fasciatus. Six monochromatic lights were tested. The effects of reduced aromatase activity, via drug inhibition, on plasma sex hormone levels(T and E2) and the preferences between sexes were investigated. The results showed that female and male fish preferred red light(around 637 nm in wavelength) and yellow light(590 nm), respectively. However, they were repelled by blue light(465 nm), purple light(405 nm), and white light with blue spectrum(447 nm), while the preference for green light(518 nm) was not obvious. Feeding the fish an experimental diet containing the aromatase inhibitor, letrozole(LZ), over 10 days,resulted in a significant reduction in the aromatase activity level in the brain and gonadal tissues, as well as in the plasma E2 level, but no change in the plasma T level was observed. Surprisingly, LZ also caused an obvious change in the color preference of lighting;i.e., females preferred blue light and males preferred red light after treatment. However, no significant changes occurred in the levels of T and E2;nor were there any in the preferences, except for the decrease in aromatase activity levels in both the females and the males fed with another experimental feed containing LZ+E2. In conclusion, the sex differences in the color preference of lighting in these fish are most likely related to the sex differences in aromatase activity and estradiol levels, but not significantly related to the testosterone levels.

关 键 词：光唇鱼 光谱偏好 性别差异 芳香化酶 睾酮 雌二醇 

分 类 号：S917[农业科学—水产科学]