血清miR-194、miR-497水平与结直肠癌患者淋巴结转移的相关性  

Correlation between serum miR-194,miR-497 levels and lymph node metastasis in patients with colorectal cancer

作　　者：梁友方[1] 贺超奇[1] 陈亮[1] LIANG You-fang;HE Chao-qi;CHEN Liang(Clinical Laboratory,the First People's Hospital of Xiaoshan District,Hangzhou,Zhejiang 311200,China)

机构地区：[1]浙江省杭州市萧山区第一人民医院检验科,浙江杭州311200

出　　处：《中国卫生检验杂志》2021年第21期2651-2654,2659,共5页Chinese Journal of Health Laboratory Technology

摘　　要：目的分析血清微小核糖核酸-194(miR-194)、微小核糖核酸-497(miR-497)表达与结直肠癌(CRC)患者淋巴结转移的关系。方法回顾分析,收集本院2018年6月-2020年6月收治46例伴有淋巴结转移CRC患者资料,纳为淋巴结转移组.另收集同期收治46例无淋巴结转移CRC患者资料,纳为无淋巴结转移组。全部CRC患者均接受血清miR-194、miR-497检测,且相关实验室检测结果病例资料等均完整;统计患者相关基线资料与血清miR-194.miR-497检测结果,分析血清miR-194.miR-497表达与CRC患者淋巴结转移的关系。结果淋巴结转移CRC患者血清miR-194.CEA.CA19-9水平均高于无淋巴结转移患者,miR-497水平低于无淋巴结转移患者,差异有统计学意义(P<0.05),组间其他基线资料比较差异无统计学意义(P>0.05);经Logistic回归分析后结果显示,miR-194、miR-497,CEA.CA19-9异常表达可能与CRC患者淋巴结转移有关,且miR-194过表达、miR-497低表达.CEA过表达,CA19-9过表达可能是CRC患者淋巴结转移的风险因子(OR>1,P<0.05),其中miR-194过表达、miR-497低表达检验所得OR值较高,可能作为独立风险因子;绘制ROC曲线发现,血清miR-194、miR-497单一及联合预测CRC患者淋巴结转移风险的AUC>0.80,均有一定预测价值。结论CRC患者淋巴结转移可能与血清miR-194过表达、miR-497低表达有关。Objective To analyze the relationship between the expression of serum microRNA-194(miR-194)and microR-NA-497(miR-497)and lymph node metastasis in patients with colorectal cancer(CRC).Methods Retrospectively analysis was performed on the data of 46 CRC patients with lymph node metastasis(lymph node metastasis group)admitted to our hospital from June 2018 to June 2020 and the data of 46 CRC patients without lymph node metastasis(non-lymph node metastasis group)treated in the same period.The detection of serum miR-194 and miR-497 were received by all CRC patients,and the relevant laboratory test results and case data were complete;the relevant baseline data and serum miR-194 and miR-497 detection results of patients were counted,and the relationship between the expression of serum miR-194 and miR-497 and lymph node metastasis in CRC patients was analyzed.Results The serum miR-194,CEA and CA19-9 levels in CRC patients with lymph node metastasis were higher than those in patients without lymph node metastasis,and the level of miR-497 was lower than that in patients without lymph node metastasis,with the differences statistically significant(P<0.05);compared with other baseline data between groups,there was no statistical significance on the difference(P>0.05).Logistic regression analysis showed that the abnormal expression of miR-194,miR-497,CEA and CA19-9 might be associated with lymph node metastasis in CRC patients,and the overexpression of miR-194,low expression of miR-497,overexpression of CEA and overexpression of CA19-9 might be risk factors of lymph node metastasis in CRC patients(OR>1,P<0.05),of which the overexpression of miR-194 and the low expression of miR-497 showed higher OR value,and it might be used as independent risk factors;the ROC curve showed that the AUC of serum miR-194 and miR-497 alone and in combination in predicting the risk of lymph node metastasis in CRC patients were greater than 0.80,which had certain predictive value.Conclusion Lymph node metastasis in CRC patients may be related to ov

关 键 词：结直肠癌 血清微小核糖核酸-194 微小核糖核酸-497 淋巴结转移 

分 类 号：R735.37[医药卫生—肿瘤]