Robust temporal changes of cellular senescence and proliferation after sciatic nerve injury  

作　　者：Yin-Ying Shen Rui-Rui Zhang Qian-Yan Liu Shi-Ying Li Sheng Yi 

机构地区：[1]Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education,Co-innovation Center of Neuroregeneration,NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products,Nantong University,Nantong,Jiangsu Province,China

出　　处：《Neural Regeneration Research》2022年第7期1588-1595,共8页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.31970968(to SYL);Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。

摘　　要：Cellular senescence and proliferation are essential for wound healing and tissue remodeling.However,senescence-proliferation cell fate after peripheral nerve injury has not been clearly revealed.Here,post-injury gene expression patterns in rat sciatic nerve stumps(SRP113121)and L4–5 dorsal root ganglia(SRP200823)obtained from the National Center for Biotechnology Information were analyzed to decipher cellular senescence and proliferation-associated genetic changes.We first constructed a rat sciatic nerve crush model.Then,β-galactosidase activities were determined to indicate the existence of cellular senescence in the injured sciatic nerve.Ki67 and EdU immunostaining was performed to indicate cellular proliferation in the injured sciatic nerve.Both cellular senescence and proliferation were less vigorous in the dorsal root ganglia than in sciatic nerve stumps.These results reveal the dynamic changes of injury-induced cellular senescence and proliferation from both genetic and morphological aspects,and thus extend our understanding of the biological processes following peripheral nerve injury.The study was approved by the Animal Ethics Committee of Nantong University,China(approval No.20190226-001)on February 26,2019.

关 键 词：bioinformatic analysis cellular senescence dorsal root ganglia P16 peripheral nerve regeneration peripheral nerve trauma PROLIFERATION rat sciatic nerves sciatic nerve crush β-galactosidase activities 

分 类 号：R446[医药卫生—诊断学] R745[医药卫生—临床医学]