明胶废水中耐钙菌株的筛选鉴定及培养条件优化  

Screening,identification and culture condition optimization of calcium resistant strain from gelatin wastewater

作　　者：白慧慧 任海伟[1,2] 任雨薇 唐多利 彭轶楠 王治业[2] 李志忠[1] BAI Huihui;REN Haiwei;REN Yuwei;TANG Duoli;PENG Yinan;WANG Zhiye;LI Zhizhong(School of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050,China;Key Laboratory of Microbial Resources Exploitation and Application of Gansu Province,Institute of Biology,Gansu Academy of Sciences,Lanzhou 730099,China)

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃兰州730050 [2]甘肃省科学院生物研究所甘肃省微生物资源开发利用重点实验室,甘肃兰州730099

出　　处：《中国酿造》2021年第11期87-92,共6页China Brewing

基　　金：甘肃省科学院青年基金项目(2018QN-09);陇原青年创新创业人才项目(2019-39-GR28);兰州理工大学红柳杰出青年人才支持计划(JQ2020)。

摘　　要：从明胶加工废水样品中筛选对Ca^(2+)具有抗性的微生物菌株,结合形态特征、生理生化试验和分子生物学技术对其进行鉴定,并利用单因素试验及响应面法优化其培养条件,以期为高钙污染水体的微生物修复提供菌株资源和应用技术参考。结果表明,当Ca^(2+)含量达到50 mg/mL时,从四个样品中筛选出1株抗钙性能较好的菌株,命名为N3-1,经鉴定其为尼阿布芽孢杆菌(Bacillus niabensis)。菌株N3-1生长的最佳培养条件为:初始Ca^(2+)含量1.40 mg/L、培养温度37℃、培养基初始pH值8.3,培养时间21 h。在此优化条件下,菌悬液OD600 nm值为0.768。Ca^(2+)resistant microbial strains were screened from gelatin processing wastewater samples,and identified by morphological characteristics,physiological and biochemical experiments and molecular biology methods.Its culture conditions were optimized by single factor test and response surface method,in order to provide strain resources and application technology references for the microbial remediation of high calcium polluted water.The results showed that when the Ca^(2+)contents reached 50 mg/ml,a strain with better calcium resistance was selected from the 4 samples,numbered N3-1,and identified as Bacillus niabensis.The optimal culture conditions for the growth of strain N3-1 were as follows:initial Ca^(2+)content 1.40 mg/L,culture temperature 37℃,medium initial pH 8.3 and time 21 h.Under the optimal conditions,OD600 nm value of the bacterial suspension was 0.768.

关 键 词：明胶废水 耐钙菌株 筛选鉴定 培养条件 响应面优化 

分 类 号：Q939.9[生物学—微生物学]