表面增强拉曼光谱结合主成分分析快速筛查食品接触材料中多环芳烃  

Rapid Screening of Polycyclic Aromatic Hydrocarbons in Food Contact Materials by Surface-enhanced Raman Spectroscopy Combined with Principal Component Analysis

作　　者：葛琨 何致峰 林佳娜 胡玉玲[1] 李攻科[1] GE Kun;HE Zhi-feng;LIN Jia-na;HU Yu-ling;LI Gong-ke(School of Chemistry,Sun Yat-sen University,Guangzhou 510275,China)

机构地区：[1]中山大学化学学院,广东广州510275

出　　处：《分析测试学报》2021年第11期1588-1595,共8页Journal of Instrumental Analysis

基　　金：国家重点研发计划(2018YFC1603201,2018YFC1603200)。

摘　　要：建立了表面增强拉曼/主成分分析快速筛查食品接触材料中4种多环芳烃的分析方法。采用纳米银溶胶作为增强基底,碘化钾为絮凝剂,实现了4种多环芳烃(芘、荧蒽、苯并[b]荧蒽、苯并[k]荧蒽)的表面增强拉曼分析。针对食品接触材料中4种多环芳烃拉曼谱峰重叠难以鉴别区分的问题,采用主成分分析法分别对同浓度多环芳烃、不同浓度多环芳烃以及多环芳烃混合样品进行分析。结果表明,4种多环芳烃均可得到较好的鉴别。该方法成功用于食品接触材料迁移液中4种多环芳烃的快速筛查。Analysis of polycyclic aromatic hydrocarbons(PAHs)has drawn great attention due to their high bio-accumulative,carcinogenic and teratogenic properties.PAHs might migrate to foods through contaminated food contact materials(FCMs),causing huge potential damages to human health.It is very important to screen PAHs in FCMs with high rapidity and accuracy.Surface-enhanced Raman spectroscopy(SERS)is a promising optical tool for contaminants screening due to its unique properties of rapidity,sensitivity and molecule fingerprint characteristic.However,PAHs is a class of pollutants including over 200 chemicals with two or more benzene rings,meaning the similar structure and overlapping of characteristic Raman peaks.Principal component analysis(PCA)is an ideal data processing method,which could be used to extract useful information from large amount of data by dimensionality reduction process.Researchers have developed methods for identifying chemicals with similar structure by SERS combined with PCA method,obtaining good segregation and identification results.In this paper,a simple analytical method was established for rapid screening of four PAHs,i.e.pyrene(Pyr),fluoranthene(FlA),benzo[b]fluorathene(BbF)and benzo[k]fluorathene(BkF)in FCMs of polyethylene terephthalate(PET)and polypropylene(PP)based on the combination of SERS and PCA.Ag nanoparticles(AgNPs)were mixed with inorganic salt of potassium iodide(KI)to form the aggregated AgNPs with denser“hot spot”,which were used as the enhanced substrate for SERS analysis with high sensitivity.In order to obtain the optimum sensitivity,some analytical parameters including inorganic salt,concentration of KI,incubation time and volume ratio of KI∶BbF∶AgNPs were optimized.Under the optimum conditions,the Raman characteristic peak of Pyr,FlA,BbF and BkF were recorded and sorted for next PCA analysis.Afterwards,PCA method was adopted for the rapid identification of four PAHs to overcome the overlapping of characteristic peaks with similar structure.Results showed that the f

关 键 词：表面增强拉曼光谱 主成分分析 食品接触材料 多环芳烃 快速筛查 

分 类 号：O657.3[理学—分析化学] TS201.6[理学—化学]