优化液相色谱法测定茶枯粉中三种防腐剂含量的方法  

Optimization of the Chromatography Method for the Determination of Three Preservatives in Tea Seed Powder

作　　者：张婧婧 刘纲勇[3] Zhang Jingjing;Liu Gangyong(Zhangzhou College of Science and Technology,Zhangzhou 363000;Leisure Food Industrial Technical Research Institute of Zhangzhou,Zhangzhou 363200;Guangdong Vocational College of Food and Drug,Guangzhou 510002,China)

机构地区：[1]漳州科技职业学院,福建漳州363000 [2]漳州市休闲食品产业技术研究院,福建漳州363200 [3]广东食品药品职业学院,广东广州510002

出　　处：《广东化工》2021年第19期191-192,200,共3页Guangdong Chemical Industry

基　　金：福建省中青年教师教育科研项目(JAT171240);福建省星火科技项目(2021S0051)。

摘　　要：目的:优化液相色谱法测定茶枯粉中三种防腐剂含量的分析方法。方法:以山梨酸、苯甲酸与糖精钠为标准物质,Syncronis C18(250×4.6 mm,5μm)为分离柱,测定波长为254 nm,3%甲醇与97%20 mmo/L的乙酸铵溶液为流动相。茶枯粉经过正己烷破脂,蛋白剂沉淀的测定条件下,以外标法定量。结果防腐剂的加标回收率在95%~108%之间,方法检出限在3.87~4.39 mg/kg之间。结论:该方法操作过程简单,结果重现性好,灵密度高,适用于茶枯粉中三种防腐剂含量的测定。objective to optimize the analytical method for the determination of three preservatives in tea seed powder.Methods sorbic acid,benzoic acid and saccharin sodium were used as reference materials,Syncronis C18(250×4.6 mm,5μm)was used as separation column,the wavelength was 254 nm,3% methanol and 97% 20 mmol/L ammonium acetate solution were used as mobile phase.Tea seed powder after n-hexane break fat,protein agent precipitation of the determination of the conditions,the external standard method quantitative.The results showed that the recovery of preservatives was between 95% and 108%,and the detection limit was between 3.87 mg/kg and 4.39 mg/kg.Conclusion:The method is simple,reproducible and suitable for the determination of three preservatives in tea seed powder.

关 键 词：优化 液相色谱法 茶枯粉 山梨酸 苯甲酸 糖精钠 

分 类 号：TQ[化学工程]