植物油基金属加工液中的生物抑菌技术研究  

Study on a New Biological Antibacterial Technology in Vegetable Oil⁃based Metalworking Fluids

作　　者：程慧杰 赵朋飞 李小磊 戴媛静 CHENG Huijie;ZHAO Pengfei;LI Xiaolei;DAI Yuanjing(Research Center of Lubrication Technology,Tianjin Research Institute for Advanced Equipment of Tsinghua University,Tianjin 300300,China;Ji Hua Laboratory,Foshan Guangdong 528200,China;State Key Laboratory of Tribology,Tsinghua University,Beijing 100084,China)

机构地区：[1]清华大学天津高端装备研究院润滑技术研究所,天津300300 [2]季华实验室,广东佛山528200 [3]清华大学摩擦学国家重点实验室,北京100084

出　　处：《润滑与密封》2021年第11期122-131,共10页Lubrication Engineering

基　　金：国家重点研发计划项目(2018YFB2002204;2020YFB2010600;2020YFA0711000);国家自然科学基金项目(51805291);贵州省科技重大专项(黔科合重大专项字[2019]3016号);国防基础科研项目(JCKY2020110B007).

摘　　要：为了在减少使用甚至零添加化学杀菌剂的前提下延缓金属加工液的腐败,提出一种全新的生物抑菌技术。首先于现有使用工况下的金属加工液体系中提取一株优势菌——铜绿假单胞菌Y1,并对优势菌Y1的生长特性进行研究,然后分析Y1在不同试验条件下的抑菌效果。研究结果表明:Y1的最适生长温度为40℃、pH值为6.0;Y1对BK、WSplus等杀菌剂具有良好的耐受性;在混菌共培试验、强制腐败试验以及实际应用现场模拟试验中,Y1均表现出良好的抑菌效果,能够较好抑制植物油基金属加工液1000Ti中主要腐败菌F1、F2和F3的滋生,并且配合少量杀菌剂使用后,其抑菌效果更佳;同时,Y1的加入不会对金属加工液pH值、防锈性能和润滑性能造成不良影响,研究结果可为环保型金属加工产品的开发提供全新的设计思路。In order to control the decomposition of metalworking fluids under the premise of reducing or even not using chemical fungicides,a new biological antibacterial technology was proposed.Firstly,a kind of pseudomonas aeruginosa(Y1)was extracted from the metal working fluid in service,and the growing characteristics of the dominant microorganism Y1 were studied,and then the antibacterial effect of Y1 under different experimental conditions was analyzed.The results show that the optimum temperature for Y1 growing is 40℃and the optimal pH value is 6.0.The microorganism Y1 has good tolerance to BK,WSplus and other chemical fungicides.In the mixed bacteria co⁃cultivation test,the forced spoilage test and the actual application field simulation test,the mciroorganism Y1 shows good antibacterial effects,which can better inhibit the growth of the main spoilage bacteria F1,F2 and F3 in 1000Ti.The antibacterial effect of Y1 can be better if it worked with a small amount of chemical fungicide.At the same time,the addition of Y1 do not adversely affect the pH val⁃ue,rust resistance and lubrication properties of the metal working fluid.The research results can provide a new design idea for the development of environment⁃friendly metal processing products.

关 键 词：生物抑菌 铜绿假单胞菌 金属加工液 腐败控制 植物油 

分 类 号：TH117[机械工程—机械设计及理论]