棉花中5-羟色胺含量的时空动态及其对棉铃虫生长发育的影响  

Temporal and spatial dynamics of 5-hydroxytryptamine content in cotton and its effects on the growth and development of cotton bollworm Helicoverpa armigera

作　　者：孙佩瑶 滕东 吕贝贝 张航 张永军[1] Sun Peiyao;Teng Dong;LüBeibei;Zhang Hang;Zhang Yongjun(State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家实验室,北京100193

出　　处：《植物保护学报》2021年第5期1075-1080,共6页Journal of Plant Protection

基　　金：国家自然科学基金(31772176,31972338)。

摘　　要：为明确棉花中5-羟色胺含量的时空动态及其对棉铃虫Helicoverpa armigera生长发育的影响,采用高效液相色谱方法检测不同棉花品种(系)中5-羟色胺含量的时空动态,并以添加外源5-羟色胺的人工饲料饲喂棉铃虫,分析5-羟色胺对其生长发育的影响。结果表明,不同棉花品种(系)叶片中5-羟色胺的含量为5.0~20.8μg/g,其中蕾期与花期棉株叶片中5-羟色胺的含量均高于苗期与铃期棉株叶片,除个别品种(系)外差异均达显著水平。饲喂添加外源5-羟色胺的人工饲料可导致棉铃虫化蛹提前、发育历期缩短以及蛹重增加。10μg/g和100μg/g的5-羟色胺对棉铃虫幼虫的死亡率或没有影响或降低死亡率,但是极高浓度(500μg/g和1000μg/g)的5-羟色胺能够提高棉铃虫幼虫的死亡率。表明常规棉花蕾期与花期较高含量的5-羟色胺可能有利于棉铃虫的生长发育,在抗虫品种(系)培育中可将低5-羟色胺含量作为抗虫种质资源的参考指标。In order to clarify the temporal and spatial dynamics of 5-hydroxytryptamine(5-HT)content in cotton and its effects on the growth and development of cotton bollworm Helicoverpa armigera,a high performance liquid chromatography(HPLC)method was established for detecting 5-HT in cotton.Based on the HPLC method,the temporal and spatial dynamics of 5-HT content in different varieties(lines)of cotton were determined.In addition,exogenous 5-HT was added to artificial diets to feed larvae of cotton bollworm,and its effect on the growth and development of this insect was investigated.The results showed that the content of 5-HT in cotton leaves ranged from 5.0μg/g to 20.8μg/g,and the contents during budding stage and flowering stage were significant higher than those during seedling and boll-setting stages.Addition of exogenous 5-HT resulted in earlier pupation of the cotton bollworm,shortened development period and increased pupal weight.The 5-HT at 10μg/g and 100μg/g did not affect or reduce the mortality of cotton bollworm larvae,but extremely high concentrations of 5-HT(500μg/g and 1000μg/g)increased the mortality of cotton bollworm larvae.The study indicated that the higher content of 5-HT at the budding and flowering stage of conventional cotton might be beneficial to the growth and development of cotton bollworm.In the breeding of insect-resistant varieties(lines),lower 5-HT content can be used as a reference index for insect-resistant germplasm resources.

关 键 词：棉花 5-羟色胺 时空动态 抗虫性 

分 类 号：S435.622.3[农业科学—农业昆虫与害虫防治]