入侵性南美番茄潜叶蛾高效药剂筛选及其抗性基因突变检测  

Screening of Efficient Insecticide against Invasive Tuta absoluta(Meyrick)and Detection of Its Resistance Gene Mutation

作　　者：王少丽[1] 史彩华[1] 徐丹丹[1] 张友军[1] WANG Shaoli;SHI Caihua;XU Dandan;ZHANG Youjun(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《中国蔬菜》2021年第11期33-36,共4页China Vegetables

基　　金：中国农业科学院基本科研业务费所级统筹项目(IVF-BRF2018015);国家现代西甜瓜产业技术体系项目(CARS-25);蔬菜有害生物控制与优质栽培北京市重点实验室项目。

摘　　要：南美番茄潜叶蛾〔Tuta absoluta(Meyrick)〕是近年来新入侵我国的危险性外来有害生物。采用改进的带虫叶片浸渍法评价7种药剂田间剂量对入侵我国新疆伊宁县的南美番茄潜叶蛾幼虫的致死效果,并对南美番茄潜叶蛾与菊酯类药剂抗性相关的钠离子通道击倒抗性(knock-down resistance,kdr)基因的点突变进行检测。结果表明,1.8%阿维菌素水乳剂、24%溴虫腈悬浮剂和5%氯虫苯甲酰胺悬浮剂对南美番茄潜叶蛾表现出极高的毒力活性,试虫死亡率达100%;其次为5%甲氨基阿维菌素苯甲酸盐水分散粒剂和6%乙基多杀菌素悬浮剂,试虫死亡率达90%以上;苏云金芽胞杆菌(Bt)对南美番茄潜叶蛾的毒力活性较低,4.5%高效氯氰菊酯乳油的毒力活性极低。kdr基因突变位点M918T、T929I和L1014F的突变频率分别为30%、75%和100%,这与菊酯类杀虫剂对该虫表现极低毒力活性的结果一致。该研究结果可为化学防控入侵我国新疆的南美番茄潜叶蛾提供科学指导。South American tomato leaf miner,Tuta absoluta(Meyrick)is an alien dangerous pest recently invade into China.This study evaluated the lethal effects of 7 insecticides field dose on larva of T.absoluta invaded into Yining County of Xinjiang Autonomous Region by modified insect blade impregnation method;and conducted detection on point mutations of sodium channel kdr gene associated with pyrethroid insecticide in T.absoluta.The results showed that 1.8%abamectin EW,24%chlorfenapyr SC and 5%chlorantraniliprole SC had sky-high virulence activity for T.absoluta larva with 100%lethality,followed by 5%emamectin benzoate WG and 6%spinetoram SC with over 90%lethality.However,the virulence activity of Bacillus thuringiensis(Bt)for T.absoluta was lower and 4.5%efficient beta-cypermethrin EC had ultra low virulence activities.The mutation frequency of kdr gene mutation points M918T,T929I and L1014F were 30%,75%and 100%,respectively,consistent to the ultra low activities of pyrethroid insecticide.This results have provided scientific guidance for the chemical prevention and control of T.absoluta invaded into Xinjiang Autonomous Region,China.

关 键 词：南美番茄潜叶蛾 药剂筛选 抗性基因 突变检测 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]