远端肢体缺血预处理对大鼠心脏骤停后肠道屏障功能的影响  

Effects of Remote Limb Ischemic Preconditioning on Intestinal Barrier Function in Cardiac Arrest Rats

作　　者：顾明明 谭焱 张瑜 朱水波 朱豫 朱健 吴银丹 高旭辉 GU Mingming;TAN Yan;ZHANG Yu;ZHU Shuibo;ZHU Yu;ZHU Jian;WU Yindan;GAO Xuhui(Department of Cardiothoracic Surgery,General Hospital of Central Theater Command,Wuhan Hubei 430070,China)

机构地区：[1]中部战区总医院心胸外科,湖北武汉430070

出　　处：《华南国防医学杂志》2021年第3期164-167,175,共5页Military Medical Journal of South China

摘　　要：目的研究远端肢体缺血预处理(remote limb ischemic preconditioning, RLIP)对心肺复苏(cardiopulmonary resuscitation, CPR)后的肠道屏障功能(intestinal barrier function, IBF)障碍是否具有保护作用。方法雄性成年SD大鼠30只,体质量250~300 g,随机分为Control组、CPR组、RLIP组3组,每组各10只。Control组未建立CPR模型和RLIP模型,CPR组采取改良Utstein模型,采取气道梗阻法创设心脏骤停-复苏模型。RLIP组首先完成RLIP,间隔24 h建立心脏骤停-复苏模型。大鼠复苏成功后,间隔12 h处死大鼠,记录肠道损伤Chiu病理评分(Chiu′s评分),测定复苏后大鼠血浆二胺氧化酶(diamine oxidase, DAO)及肠脂肪酸结合蛋白(intestinal fatty acid binding protein, IFABP)水平。结果 CPR组和RLIP组的Chiu评分较Control组明显增高(P<0.05),RLIP组的Chiu评分较CPR组明显增高(P<0.05)。CPR组及RLIP组的DAO值均明显低于Control组(P<0.05),RLIP组的DAO值明显高于CPR组(P<0.05)。CPR组及RLIP组的IFABP值较Control组明显增高(P<0.05),RLIP组的IFABP值明显低于CPR组(P<0.05)。结论 RLIP可改善大鼠CPR后IBF损伤的相关指标,进而推论RLIP对大鼠CPR后IBF的损伤有一定保护作用。Objective To investigate whether remote limb preconditioning(RLIP) has a protective effect on intestinal barrier dysfunction after cardiopulmonary resuscitation(CPR).Methods A total of 30 male adult SD rats, weighing 250-300 g, were randomly divided into Control group, CPR group and RLIP group, with 10 rats in each group.The Control group did not establish the CPR model and the RLIP model. The CPR group adopted the modified Utstein model and adopted the airway obstruction method to create the cardiac arrest-resuscitation model. The RLIP group first completed the RLIP, and established the cardiac arrest and resuscitation model at 24-hour interval. The intestinal injury Chiu pathological score(Chiu′s score) and plasma diamine-oxidase(DAO) and intestinal fatty acid binding protein(IFABP) were measured 12 hours after resuscitation.Results The Chiu score of CPR group and RLIP group was significantly higher than that of Control group(P<0.05). The Chiu score of RLIP group was significantly higher than that of CPR group(P<0.05).The DAO value of CPR group and RLIP group was significantly lower than that of Control group(P<0.05). The DAO value of RLIP group was significantly higher than that of CPR group(P<0.05). The IFABP level of CPR group and RLIP group was significantly higher than that of Control group(P<0.05). The IFABP level of RLIP group was significantly lower than that of CPR group(P<0.05).Conclusion RLIP can improve the related indexes of intestinal barrier function(IBF) injury after CPR in rats, and it is inferred that RLIP has a certain protective effect on IBF injury after CPR in rats.

关 键 词：远隔肢体缺血预处理 心肺复苏 屏障功能 二胺氧化酶 肠脂肪酸结合蛋白 

分 类 号：R541.6[医药卫生—心血管疾病] R574.4[医药卫生—内科学]