Aurora-A激酶对急性胰腺炎大鼠肺脏损伤的修复作用研究  

Study on the Repairing Effect of Aurora-A Kinase on Lung Injury in Rats with Acute Pancreatitis

作　　者：解凤妮 李云龙 岳蓉 李彩茹 文雯 闫黎娜[3] 周晓燕 XIE Feng-ni;LI Yun-long;YUE Rong;LI Cai-ru;WEN Wen;YAN Li-na;ZHOU Xiao-yan(Digestive Care Unit,First Affiliated Hospital of Air Force Medical University,Xi'an,Shaanxi,710032,China;Emergency Department,Xi'an Daxing Hospital,Xi'an,Shaanxi,710016,China;Department of Neonatology,Second Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xianyang,Shaanxi,712000,China;Department of Oncology,Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xianyang,Shaanxi,712000,China)

机构地区：[1]空军医科大学第一附属医院消化监护室,陕西西安710032 [2]西安大兴医院急诊科,陕西西安710016 [3]陕西中医药大学第二附属医院新生儿科,陕西咸阳712000 [4]陕西中医药大学附属医院肿瘤二科,陕西咸阳712000

出　　处：《现代生物医学进展》2021年第18期3422-3425,3435,共5页Progress in Modern Biomedicine

基　　金：陕西省中医管理局中医药科研项目(JCPT004)。

摘　　要：目的:探讨与研究Aurora-A激酶对急性胰腺炎大鼠肺脏损伤的修复作用。方法:36只雄性SD大鼠均分为三组:对照组、模型组与Aurora-A组。对照组进行假手术操作,模型组建立急性胰腺炎模型后给予注射等量生理盐水治疗,Aurora-A组建立急性胰腺炎模型后给予阴茎背静脉注射鼠Aurora-A类因子-MLN8054 10 mg/kg治疗,记录大鼠肺脏损伤的修复情况。结果:造模过程中无大鼠死亡情况发生,模型组与Aurora-A组造模后2 w与4 w的肺组织病理评分、血清中性粒细胞弹性蛋白酶(neutrophil elastase,NE)与髓过氧化物酶(myeloperoxidase,MPO)含量、肺组织W/D、肺组织蛋白激酶B(AKT)、细胞外信号调节激酶1(ERK1)蛋白相对表达水平都高于对照组(P<0.05),Aurora-A组少于模型组(P<0.05)。结论:Aurora-A激酶在急性胰腺炎大鼠的应用能抑制Akt/ERK信号通路激活,减少血清NE与MPO的表达,从而促进肺脏损伤修复。Objective:To explore and study the repair effect of Aurora-A kinase on lung injury in rats with acute pancreatitis.Methods:36 cases of male SD rats were equally divided into three groups-control group,model group and Aurora-A group.The control group were given underwent sham operation,the model group were treated with the same amount of normal saline after the acute pancreatitis model were established,and the Aurora-A group were treated with the mouse Aurora-A factor-MLN805410 mg/kg after the acute pancreatitis model were established.Recorded the repair effects of rat lung injury.Results:There were no rats died during the modeling process.The lung tissue pathology scores,serum neutrophil elastase(NE)and myeloperoxidase(MPO),lung tissue W/D,lung tissue protein kinase B(AKT),and extracellular signal-regulated kinase 1(ERK1)protein in the model group and Aurora-A group at 2 weeks and4 weeks after modeling were higher than those in the control group(P<0.05),the Aurora-A group were less than the model group(P<0.05).Conclusion:The application of Aurora-A kinase in acute pancreatitis rats can inhibit the activation of Akt/ERK signaling pathway,reduce the expression of serum NE and MPO,and promote lung injury repair.

关 键 词：AURORA-A激酶 急性胰腺炎 肺脏损伤 髓过氧化物酶 细胞外信号调节激酶1 

分 类 号：R-33[医药卫生] R576