不同介入时间和不同疗程高压氧治疗对脑出血模型大鼠动物学行为及促血管新生因子表达的影响  

Effects of Different Intervention Time and Course of Hyperbaric Oxygen Therapy on Animal Behavior and the Expression of Angiogenic Factors in Rats with Cerebral Angiogenesis

作　　者：权瑜[1] 王举波[1] 程格庆 李娜[1] 李小冬 屈佩 王茂德[2] QUAN Yu;WANG Ju-bo;CHENG Ge-qing;LI Na;LI Xiao-dong;QU Pei;WANG Mao-de(Department of neurosurgery,the 2nd Affiliated Hospital of Xi’an Jiaotong University,Xi'an,Shaanxi,710004,China;Department of neurosurgery,the 1st Affiliated Hospital of Xi’an Jiaotong University,Xi'an,Shaanxi,710061,China)

机构地区：[1]西安交通大学第二附属医院神经外科,陕西西安710004 [2]西安交通大学第一附属医院神经外科,陕西西安710061

出　　处：《现代生物医学进展》2021年第21期4045-4050,共6页Progress in Modern Biomedicine

基　　金：陕西省自然科学基础研究计划(2017JC2-09)。

摘　　要：目的:探讨不同介入时间和不同疗程高压氧治疗对脑出血大鼠动物学行为及促血管新生因子表达的影响。方法:240只SD大鼠随机分为假手术组、脑出血组、高压氧组,每组各80只。采用胶原酶诱导建立脑出血大鼠模型,建模成功后根据高压氧介入时间分为6 h介入组、1 d介入组、2 d介入组、3 d介入组等亚组。分别治疗1周、2周、3周、4周,采用Longa评分法、平衡木评分法、Berderson评分法评估大鼠动物学行为,采用qRT-PCR检测大鼠脑组织血管内皮生长因子(VEGF)、缺氧诱导因子-1α(HIF-1α)m RNA表达。结果:不同介入时间、不同疗程,脑出血组、高压氧组脑出血大鼠动物学行为评分、脑组织VEGF m RNA与HIF-1αm RNA表达均高于假手术组(P<0.05)。治疗1周、2周、3周、4周,不同介入时间高压氧组脑出血大鼠动物学行为评分低于脑出血组,脑组织VEGF m RNA、HIF-1αm RNA表达高于脑出血组(P<0.05)。高压氧介入时间越早,治疗疗程越长,脑出血大鼠动物学行为评分越低,脑组织VEGF m RNA、HIF-1αm RNA表达越高,差异均有统计学意义(P<0.05)。结论:高压氧治疗能够改善脑出血大鼠动物学行为、促进促血管新生因子表达,且以脑出血后6 h介入、疗程4周效果最佳。Objective:To investigate the effects of different intervention time and different courses of hyperbaric oxygen therapy on animal behavior and the expression of angiogenic factors in rats with cerebral angiogenesis.Methods:240 SD rats were randomly divided into sham operation group,cerebral hemorrhage group and hyperbaric oxygen group with 80 rats in each group.The rat model of cerebral angiogenesis was established by collagenase induction.After successful modeling,according to the time of hyperbaric oxygen intervention,the rats were divided into several subgroups of 6-hour intervention group,1-day intervention group,2-day intervention group and 3-day intervention group.1 week,2 weeks,3 weeks,4 weeks after treatment,Longa score,Balance beam score and Berderson score were used to evaluted animal behavior of the rats,qRT-PCR was used to detect the m RNA expressions of vascular endothelial growth factor(VEGF)and hypoxia inducible factor-1α(HIF-1α)in rats’brain.Results:With different intervention time and different course of treatment,the animal behavior score,VEGF m RNA and HIF-1αm RNA expression in brain tissue of rats in cerebral hemorrhage group and hyperbaric oxygen group were higher than those in sham operation group(P<0.05).After treatment for 1 week,2 weeks,3 weeks and 4 weeks,the animal behavior score of rats with cerebral hemorrhage in hyperbaric oxygen group with different intervention time was lower than that in cerebral hemorrhage group,and the expression of VEGF m RNA and HIF-1αm RNA in brain tissue was higher than that in cerebral hemorrhage group(P<0.05).The earlier the time of hyperbaric oxygen intervention,the longer the course of treatment,the lower the score of animal behavior,and the higher the expression of VEGF m RNA and HIF-1αm RNA in brain tissue of rats with cerebral hemorrhage(P<0.05).Conclusion:Hyperbaric oxygen therapy can improve the animal behavior and promote the expression of angiogenic factors in rats with cerebral angiogenesis.When the hyperbaric oxygen therapy was started 6 h

关 键 词：脑出血 高压氧治疗 动物学行为 促血管新生因子 

分 类 号：R-33[医药卫生] R743.3