运动训练对脑缺血再灌注大鼠认知功能及前额皮层神经元核抗原和突触素1表达的效果  

Effect of Exercise on Expression of NeuN and SynapsinI in Cortex and Cognitive Function in Rats after Cerebral Ischemia-reperfusion

作　　者：凌梦钰 杨一卓 刘帅 叶超群 LING Meng-yu;YANG Yi-zhuo;LIU Shuai;YE Chao-qun(Air Force Clinical College,Anhui Medical University,Beijing 100142,China;Department of Rehabilitation,Air Force Medical Center,Beijing 100142,China)

机构地区：[1]安徽医科大学空军临床学院,北京市100142 [2]空军特色医学中心康复科,北京市100142

出　　处：《中国康复理论与实践》2021年第11期1272-1281,共10页Chinese Journal of Rehabilitation Theory and Practice

基　　金：中央军委后勤科研重点项目(No.BKJ17J006)。

摘　　要：目的观察运动训练对脑缺血再灌注损伤大鼠认知功能及前额皮层神经元核抗原(NeuN)和突触素1(SynapsinI)表达的影响,探究运动对缺血再灌注后认知功能改善的机制。方法雄性Sprague-Dawley大鼠40只随机数字表法分成假手术组(S组)、模型组(M组)、假手术跑台组(SE组)和模型跑台组(ME组),每组10只。线栓法制备左侧大脑中动脉栓塞1 h再灌注模型,SE组和ME组行跑台训练14 d。训练后,采用旷场实验和新物体识别实验评价大鼠认知功能;尼氏染色和免疫荧光染色观察大鼠前额皮质中神经细胞的数量及分布情况,通过ELISA观察血清SynapsinI表达。结果与S组相比,M组旷场实验活跃度、穿格次数、总距离、中央区域活动时间,新物体识别实验认知指数和总距离均下降(P<0.05);与M组相比,ME组旷场实验活跃度、穿格次数、总距离、中央区域活动时间及新物体识别实验认知指数和总距离均提高(P<0.05)。M组较S组尼氏体计数减少(P<0.05),排列紊乱;ME组较M组尼氏体数量增多(P<0.05);M组NeuN阳性细胞数表达较S组降低(P<0.05),ME组较M组增加(P<0.05);M组SynapsinI阳性细胞数较S组降低(P<0.05),ME组较M组增加(P<0.05);M组血清SynapsinI表达较S组降低(P<0.05),ME组较M组增加(P<0.05);行为学实验结果多与尼氏体、NeuN、SynapsinI表达量呈正相关(r>0.221,P<0.05)。结论脑缺血再灌注会导致大鼠认知功能损伤,运动训练能减轻神经损伤,改善认知功能,可能与促进NeuN和SynapsinI表达,增加前额皮质神经元和突触数量有关。Objective To observe the effect of exercise on cognitive function and the expression of NeuN and SynapsinI in prefrontal cortex of rats after cerebral ischemia-reperfusion.Methods Forty health male Sprague-Dawley rats were randomly divided into sham group(S group,n=10),model group(M group,n=10),sham&exercise group(SE group,n=10)and model&exercise group(ME group,n=10).The left middle cerebral artery was occluded for one hour and reperfused.SE and ME groups accepted treadmill training,for 14 days.The cognitive function of rats was evaluated with open field experiment and new object recognition experiment.The number and distribution of nerve cells in rat brain were observed with Nissl staining and immunofluorescence staining.The SynapsinI expression in serum was detected with ELISA.Results Compared with S group,the activity,times of crossing,length and activity time in central area of open field experiment decreased in M group,as well as the cognitive index and length of new object recognition experiment(P<0.05).Compared with M group,all the indexes increased in ME group(P<0.05).The number of Nissl bodies decreased in M group(P<0.05)and the arrangement was disordered,while the number of Nissl bodies increased in ME group(P<0.05)compared with M group.The NeuN positive cells was more in S group than in M group(P<0.05),and it was more in ME group than in M group(P<0.05);as well as that of SynapsinI.The expression of SynapsinI decreased in M group(P<0.05),and increased in ME group(P<0.05).Most results of behavioral experiments positively correlated with the expression of Nissl,NeuN and SynapsinI(r>0.221,P<0.05).Conclusion Cerebral ischemia-reperfusion can cause cognitive impairment in rats.Exercise can alleviate nerve injury and improve cognitive function,which may relate to promoting the expression of NeuN and SynapsinI in prefrontal cortex,to increase the number of neurons and synapses.

关 键 词：缺血再灌注 认知障碍 运动训练 突触素1 神经元核抗原 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]