Short-chain fatty acids can improve lipid and glucose metabolism independently of the pig gut microbiota  

作　　者：Hua Zhou Bing Yu Jing Sun Zuohua Liu Hong Chen Liangpeng Ge Daiwen Chen 

机构地区：[1]Key Laboratory of Animal Disease-Resistance Nutrition,Chengdu 611130,Sichuan,China [2]Animal Nutrition Institute,Sichuan Agricultural University,Chengdu 611130,Sichuan,China [3]Key Laboratory of Pig Industry Sciences,Rongchang 402460,Chongqing,China [4]Chongqing Academy of Animal Sciences,Rongchang 402460,Chongqing,China [5]College of Food Science,Sichuan Agricultural University,Ya’an 625014,Sichuan,China

出　　处：《Journal of Animal Science and Biotechnology》2021年第4期1414-1427,共14页畜牧与生物技术杂志（英文版）

基　　金：study was supported by National Natural Science Foundation of China(31730091);the National Key Research and Development Program of China(2017YFD0500503).

摘　　要：Background:Previous studies have shown that exogenous short-chain fatty acids(SCFAs)introduction attenuated the body fat deposition in conventional mice and pigs.However,limited studies have evaluated the effects of exogenously introduced SCFAs on the lipid and glucose metabolism independently of the gut microbiota.This study was to investigate the effects of exogenous introduction of SCFAs on the lipid and glucose metabolism in a germ-free(GF)pig model.Methods:Twelve hysterectomy-derived newborn pigs were reared in six sterile isolators.All pigs were hand-fed with sterile milk powder for 21 d,then the sterile feed was introduced to pigs for another 21 d.In the second 21-d period,six pigs were orally administrated with 25 mL/kg sterile saline per day and considered as the GF group,while the other six pigs were orally administrated with 25 mL/kg SCFAs mixture(acetic,propionic,and butyric acids,45,15,and 11 mmol/L,respectively)per day and regarded as FA group.Results:Orally administrated with SCFAs tended to increase the adiponectin concentration in serum,enhance the CPT-1 activity in longissimus dorsi,and upregulate the ANGPTL4 mRNA expression level in colon(P<0.10).Meanwhile,the mRNA abundances of ACC,FAS,and SREBP-1C in liver and CD36 in longissimus dorsi of the FA group were decreased(P<0.05)compared with those in the GF group.Besides,the mRNA expression of PGC-1αin liver and LPL in longissimus dorsi tended to(P<0.10)upregulate and downregulate respectively in the FA group.Moreover,oral administration of SCFAs tended to increase the protein level of GPR43(P<0.10)and decrease the protein level of ACC(P<0.10)in liver.Also,oral administration of SCFAs upregulated the p-AMPK/AMPK ratio and the mRNA expressions of GLUT-2 and GYS2 in liver(P<0.05).In addition,the metabolic pathway associated with the biosynthesis of unsaturated fatty acids was most significantly promoted(P<0.05)by oral administration of SCFAs.Conclusions:Exogenous introduction of SCFAs might attenuate the fat deposition and to some extent improve th

关 键 词：GERM-FREE Glucose metabolism Lipid metabolism Pig model Short-chain fatty acids 

分 类 号：S828.5[农业科学—畜牧学]